huangying 本人因实验需要,欲购买霍乱毒素(CTX)和百日咳毒素(PTX),价格公道,尽快到货,如个人实验剩余亦可,毕业将即,急急急啊! 先叩谢了!:'(:'(fyk0317 你是需要做什么实验的百日咳毒素jingdan1983 你好,谢谢你的回复,我们是做大白鼠的实验,想用百日咳毒素和霍乱毒素来激动和阻断鼠大脑纹状体和海马中的G蛋白,但是现在不知道怎么给药,以及药物的半衰期和给药浓度,所以能否请你指点一下?谢谢。因为遇到这个难题 ...
ntgxw 跨膜蛋白分为单次跨膜蛋白,多次跨膜蛋白等等,我想请问一下这个多次跨膜蛋白再怎么具体分为几次几次的呢?比如说G蛋白偶联型受体是7次跨膜蛋白,这是根据什么来的呢?谢谢!amygdala 不知道你想问什么问题,你既然已经自己给出了GPCR的结构简图,那还用问什么呢?数数GPCR在细胞膜内的结构域,不就是7个吗?pharmacologist 细胞膜上的跨膜信号传递蛋白主要有两大类一、由具有特异感受结构的通道蛋白质完成 ...
九条虫 如题,谁能给出一个定义一般核定位蛋白是不是和这个核是统一体内的呢,比如说是这个细胞内的蛋白入核要是外源的蛋白,比如细菌的蛋白可以导入细胞并通过什么机制入核吗?而这种入核的检测可以通过什么方法,主要是借助真核转染,然后看定位吗?一个单纯蛋白可不可能在不借助菌体,自动导入细胞内(据说炭疽的毒力蛋白是这样的),并可能最终入核?也就是说这蛋白本身就具有针对异种的核定位信号。可能吗?lwjssry 楼主的这个话题很有意思 ...
hbqh 求助各位推荐价格比较便宜的PKA激活剂和抑制剂,由于经费有限,查阅各文献和试剂公司,PKA激活剂和抑制剂价格都很高,哪位前辈能够推荐价格比较便宜的PKA激活剂和抑制剂。谢谢基因小子 有小包装的,你可以试一下。大约300一个吧碧云天的forskolin鞠大鹏 H89 么特异的抑制剂wzc2004 我有PKA激活剂和抑制剂,我们可以交换试剂。邮箱 ahwzc@126.comsindy5188 药物对细胞是其抑制作用的,想寻求药物作 ...
sofa623 各位老师,前辈,求教了,请问在国内和ATCC的大肠癌细胞系中,哪些是TGF-beta受体高表达,哪些是低表达呢,我自己找了很久,但是收获不大,哪位做过这方面研究的老师能不能指点一下,或者说明一下我该查哪些网站也好——我已经查阅了ATCC,上海细胞库的细胞系说明,还有pubmed上的相关文献,还是没有明确的说明的——先谢谢了sofa623 哪位老师有相关的经验,请不吝赐教,谢谢,谢谢基因小子 T47D不表达二型受体 ...
kelvin12 请问各位同行谁有多余的兔抗Paxillin和鼠抗phorsphotyrosine,能否相赠?谢谢!iammonkey kelvin12战友:你知道paxillin磷酸化的阻断剂是什么吗?正做相关东西,刚接触不太明白!磷酸化抗体 我公司有现货,具体如下:IBN4001 e-Acetylated Lysine AntibodyIBN4006 e-Acetylated Lysine Antibody-FITCIBN4010 Acetyl ...
erythrocyte2005 ECM成分如何影响已知的信号转导路径?可能的机制有哪些?最好能够举例说明下!谢谢!茯苓猪爱 推荐一篇文章:Emilin1 links TGF-beta maturation to blood pressure homeostasis.Cell. 2006 Mar 10;124(5):929-42.PMID: 16530041实验当中有所反映erythrocyte2005 谢谢茯苓猪爱战友!你也在做这方的工作么?我现在看的也是一种较新的分 ...
烟雨无心客 请教有没有做肝细胞胰岛素抵抗信号通路的战友?希望提供肝细胞胰岛素抵抗的信号通路感谢kaige88 胰岛素抵抗的经典定义是胰岛素维持正常血糖的能力下降,即一定浓度的胰岛素没有达到预期的生理效应,或组织对胰岛素的反应下降现象,临床表现为高胰岛素血症。胰岛素抵抗发生的主要部位是依赖胰岛素的葡萄糖利用器官,如骨骼肌、肝脏、脂肪组织。虽然胰岛素受体数目减少以及受体结合能力下降均可导致胰岛素抵抗,但有证据显示这可能 ...
Native Promoter Reporters Validate Transcriptional TargetsMethods in Molecular Biology v468. chapter 9Otto Schmalhofer, Simone Spaderna ,and Thomas BrabletzAbstractThe transcriptional activator b-catenin is the key mediator of the canonical Wnt signaling pathway ...
Assaying b-Catenin/TCF Transcription with b-Catenin/TCFTranscription-Based Reporter ConstructsMethods in Molecular Biology v468. chapter 8AbstractTranscription-based reporters have been instrumental in characterizing the Wnt/b-catenin signalingpa ...
Detection of b-Catenin Localization by ImmunohistochemistryMethods in Molecular Biology v468. chapter 7Abstractβ-catenin is a widely expressed 90-kDa protein with dual functions in cell adhesion and Wnt signalling. At the membrane, β-catenin forms complexes with E-cadherin ...
Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in CellsMethods in Molecular Biology v468. chapter 6AbstractThe adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers.APC is a multi-dom ...
Inhibition of Glycogen Synthase Kinase-3Methods in Molecular Biology v468. chapter 5AbstractThere are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt, Hedgehog, and insulin pathways, as well as the act ...
AbstractGlycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action of Wnts. This protein kinaseis ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling oractivation of growth factor receptor tyrosine kinases (e ...
AbstractRecombinant expression of secreted Frizzled-related proteins (sFRPs) in mammalian expression systemsis a convenient source of these proteins for biological studies. Yields of protein vary; screening of clonallines for high expression is usually worthwhile. Hep ...
Wnt proteins and their signaling cascades are involved in a wide variety of developmental processes, andderegulation of this pathway is frequently associated with tumorigenesis. Unlike many other growthfactors, Wnts long eluded biochemical purification, in large part because of their hydrophobic nature,which is imparted by one or more lipid modifications (1 – 3) . Here I describe a complete protocol thatoutlines the purification process for Wnt proteins. While this protocol has not been applied
Embryonic development of multicellular organisms is an incredibly complex process that relies heavily on evolutionarily conserved signalling pathways to provide crucial cell–cell communication. Typically, secreted signalling proteins such as Wnts, BMPs, and Hedgehogs released by one cell population will trigger concentration-dependent responses in other cells located some distance away. In adults, the same signalling pathways orchestrate tissue renewal in organs such as the intestine and ski
paulin98122站友:我做的是与细胞分化有关的MAPK/ERK通路,目的是为了探讨干细胞诱导分化后通路中P-ERK/ERK的变化以及在亚细胞定位。已经初步证明了干细胞的定向分化与MAPK/ERK通路有关,但还没有找到ERK在核内 的下游分子,有没有做过ERK通路的前辈能够指导一下。做信号通路成本真的很高,许多抗体用过后就不用了,做新的通路又要重新买,真希望做通路的朋友之间能够互通有无。 ...
1.掌握cAMP的产生及灭活机制;PKA的结构、活化机制及其作用;cAMP-PKA信号转导通路;cAMP对糖原代谢过程的调节。 2.熟悉cAMP作用的靶分子;cAMP对基因表达的调节;cGMP的产生与灭活;cGMP作用的靶分子;cGMP调节的生理功能。 一、cAMP的发现及第二信使学说 cAMP是第一个被发现的第二信使。 激素作用的第二信使学说: 胞外化学物质(第一信使)不能进入细 ...
肝癌是全球六大常见癌症和三大致死性癌症之一。 肝癌的恶性转移主要由几个原因介导的,特别是IGF及其配体、受体、底物、和配体结合蛋白。 IGF信号通路通过与细胞表面上特异性受体结合的形式在调节细胞增殖、分化、凋亡等方面起着非常重要的生理作用。 IGF系统包括配体(IGF-1IGF-2和胰岛素),受体(IGF-2R胰岛素受体IRIGF01R/IR杂交受体HR)和6个高亲和 ...