细胞培养

Day -1 Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells. Day 1 Trypsinized the cells as for normal passaging until the colonies li ...

Day -1 Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells. Day 1 Trypsinized the cells as for normal passaging until the colonies li ...

1. Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2. Sacrifice the pregnant female mouse (day 13 or 14 p.c.) by cervical dislocation. Dissect out the uterine h ...

Three weeks prior to embryonic fibroblast isolation a PGK-neo male mouse is mated to a heterozygote female. 13.5 days to 14.5 days after the plug is observed sacrifice the pregnant female either by ce ...

血清的热灭活是很多培养者感兴趣的话题，价格不菲的血清中含有诸如生长因子，维生素，氨基酸等珍贵物质，而将它们置于50℃以上的温度长达30分钟是完全没有必要的。尽管如此，在大多数实验室之中血清的热灭活还是作为常规来执行，多数实验者并没有考虑热处理对血清中的生长因子，氨基酸等成分带来的负面影响，在我们的技术热线中最常被提到的就是否该对血清进行热灭活，下面我们就对胎牛血清的热灭活进行一些探讨和解释。

培养基DMED的配置。

细胞原代和传代培养的步骤及注意事项。

细胞培养常见问题及解答

The technique described here is a slight modification (March 1997) of methods presented in: Nagy A. J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical ...

Day 0 One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in a 37oC water bath. When the last bit of ice is melted spray the vial with 70% ethanol and transfer the contents of the ...

1.Ice on tray.2. Dissection tools: 1 pair of large scissors; 2 pairs of fine scissors; 1 pair of coarse forceps; 2 pairs of fine forceps; soak in 95% EtOH.&nb ...

以下是根据NIH老年病研究所提供的英文的R1胚胎干细胞培养protocol整理而成。根据经验作部分修改。 1、一般培养：保持胚胎干细胞处于未分化状态 培养基细胞复苏冻存细胞明胶包被&n ...

Passaging cells Pour out media from flasks. Wash with Hanks. 5 ml per flask. Tilt around then dump. Add 4 ml of Trypsin / EDTA to each flask. Tip then bang. Add 20% FBS NCTC media and tilt. 4 ml p ...

Materials Chick embryo (approximately 8 days old) 70% (v/v) ethanol for swabbing Sterile scissors forceps and probes Sterile petri plates Phosphate buffered saline (PBS) Trypsin cold sterilized in a 1 ...

Whole mount staining. The protocol given uses a single primary antibody with nuclear counterstaining but it can be extended to double antibody staining. It requires an inverted microscope with fluores ...

1.Basic Techniques - The 'Do's and Don'ts' of Cell Culture Given below are a few of the essential "do's and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protecti ...

How to thaw serum: Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum at -70º C as it does not prolong ...

细胞培养常见问题及解答

Introduction A major advance in our knowledge of cells came about with the ability to maintain them in continous culture. Prokaryotes have been cultured for a relatively long time but eukaryotic cultu ...

62 氯化钠结晶紫增菌液成分:蛋白胨 20g氯化钠 40g0.01%结晶紫溶液 5mL蒸馏水 1000mLpH9.0制法:除结晶紫外，其他按上述成分:配好，加热溶解。约加30%氢氧化钾溶液4。5mL，校正pH。加热煮沸，过滤。再加入结晶紫溶液，混合后分装试管。121℃高压灭菌15min。 63 氯化钠蔗糖琼脂成分:蛋白胨 10g牛肉膏 10g氯化钠 50g蔗糖 10g琼脂 18g0.2%溴麝香草酚 ...