细胞培养

MS MEDIUM FOR ARABIDOPSIS To 990 ml H2O add: Sucrose ........... 10.0 g MOPS .............. 0.5 g Agar .............. 8.0 g Adju ...

最近看大家常提到支原体污染，就翻了翻书，做了些笔记，现在贴出来和大家分享： 支原体是一种大小介于细菌和病毒之间(最小直径0．2um)并独立生活的微生物．约有1%可通过滤菌器。支原体无细胞壁形态呈高度多形性．可为圆形、丝状或梨形。 支原体形态多变，在光境下不易看清内部结构。电镜下观察支原体膜为三层结构．其中央有电干密度大的密集颗粒群或丝状的中心束。支原体多吸附或散在于细胞表面和细胞之间。横断面与细 ...

This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specifi ...

1.Add 100 l of well-mixed anticoagulated whole blood to the bottom of a labeled tube. 2.Add the appropriate primary antibody to each tube. If using unlabeled antibody a titration is suggested. Conjug ...

A general protocol for staining cell for cytometry analysis General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Di ...

When an irreplaceable culture becomes contaminated researchers may attempt to eliminate or control the contamination. First determine if the contamination is bacteria fungus mycoplasma or yeast. Isola ...

Authentication Techniques Whatever the scope of work to be carried out it is important to know that the work is being conducted using the correct reagents. This is no less important for cell cultures ...

We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result most of our incub ...

1. embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. 2. metanephroi and associated ureteric buds are microdissected and placed in holding medium (L15 medium supplemented ...

embryos are dissected from timed-pregnant mice from 10.5-11.5 d.p.c. limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ serum extender) (all me ...

Isolation of Lung Bud Endoderm What you need: E11-12 mouse embryos DMEM with 5% fetal bovine serum petri dishes for dissections and washes Tyrode-Ringer's solution pH 7.6-7.7 (recipe below) pancr ...

肿瘤细胞在组织培养中占有核心的位置，首先癌细胞是比较容易培养的细胞。当前建立的细胞系中癌细胞系是最多的。另外肿瘤对人类是威胁最大的疾病。肿瘤细胞培养是研究癌变机理、抗癌药检测、癌分子生物学极其重要的手段。肿瘤细胞培养对阐明和解决癌症将起着不可估量的作用。

培养细胞的完全培养基由基础培养基（如MEM）和添加剂（如血清或无血清培养用的某些确定的激素及生长因子）组成，下面介绍培养基及培养基中添加的成分的特性和用途。

Phosphate-buffered saline (PBS) (0.01 M Na2HPO4 0.15 M NaCl pH 7.4):sodium phosphate dibasic (Na2HPO4) 0.87 gsodium phosphate m ...

Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days they should be centrifuged at a 64.4 xg on the IEC cli ...

Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 ...

293细胞是腺病毒载体的包装细胞。腺病毒是继逆转录病毒后用于基因治疗研究的热门载体。直接将腺病毒载体导入人体内表达目的基因，治疗恶性肿瘤，心血管疾病或一些遗传疾病已取得可喜进展；利用腺病毒载体在包装细胞 293中表达分泌性蛋白质，如蛋白酪氨酸激酶 1C等亦成为生产重组蛋白的一条途径。因此完善 293细胞的大规模培养技术具有越来越重要的市场意义。哺乳细胞的大规模培养方式有三种：贴壁培养，微载体培养， ...

支原体菌株来源：M.Arginini ATCC23838 精氨酸支原体M.FermentaneATCC19989发酵支原体M.SalivariumATCC23064唾液支原体 ...

细胞株若受到细菌、真菌、支原体、或是特定病毒等之污染时，会严重的影响实验的结果。而细菌、真菌等之微生物污染时，较易自培养基等的外观变化察觉。但是若受到支原体之污染时，细胞之外观较无明显变化，但是其污染会造成细胞之生长速率缓慢、细胞产生病变之型态改变等等变化。 各国细胞库支原体污染的统计表，如下：国家　 受支原体污染(%) 报告年度USA－FDA 15 1993(past 30 years)USA－ ...

Day -1 Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells. Day 1 Trypsinized the cells as for normal passaging until the colonies li ...