琼脂糖凝胶电泳

相关专题 一、探针的标记 1.如下设置探针标记的反应体系： (1)待标记探针(1.75 pmol/微升)：2微升。 (2)T4 Polynucleotide Kinase Buffer(10X)：1微升。 (3)Nuclease-Free Water：5微升。 (4)atp(3 000 Ci/mmol at 10 mCi/ml)：1微升。 (5)T4 Polynucleotide Kinase(5-10 u/微升)：1微升。 (6)总体积10微升。 (7)按照上述反应体系依次加入各种试剂，加入同位素后，Vortex混匀， ...

相关专题 1. 什么是凝胶迁移或电泳迁移率实验? 凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术，可用于定性和定量分析。这一技术最初用于研究DNA结合蛋白，目前已用于研究RNA结合蛋白和特定的RNA序列的相互作用。 通常将纯化的蛋白和细胞粗提液和32P同位素标记的DNA或RNA探针一同保温，在非变性的聚丙烯凝胶电泳上，分离复合物和非结合的探针。DNA复合物或RNA复合物比 ...

相关专题 组别1为阴性对照;组别2中生物素标记的检测基因(LXRE2)与蛋白结合，出现条带迁移;组别3中没有标记的基因(LXRE)竞争性与蛋白结合，LXRE2条带不迁移;组别4中对LXRE进行突变，LXRE2条带重新发生迁移。从实验结果可以明确LXRE2为与调控蛋白特异结合的位点。 标签: 广州赛诚生物 EMSA 转录调控 作者：广州赛诚生物 点击：次

1. itraq的原理是什么？ Itraq的检测可以做这样的比喻，蛋白被打成肽段后，被平均的铺在了一个有384个孔的平板上，然后机器会从每个孔中选取浓度在前5名的蛋白进行鉴定和比对，所以，如果假设每个一个样本中有100个蛋白，他们的浓度均为1%，那么得到的结果就是蛋白的得分低，但是鉴定的蛋白数量会很多，可以达到90以上；如果一个样本中有100个蛋白，有3个蛋白的含量分别为30、20、 ...

Methylene Blue DNA staining protocolProtocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0. ...

1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the following:0.40 g Agarose4.00 mL 50X TBE (4X TAE fina ...

Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established te ...

Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established te ...

Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially ...

ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:Use 0.8% agarose (w/v) for high molecular weight DNA fragments and 1 - 1.2% for smaller DNA fragments. 0.5% gels are very flims ...

Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially ...

ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:Use 0.8% agarose (w/v) for high molecular weight DNA fragments and 1 - 1.2% for smaller DNA fragments. 0.5% gels are very flims ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively charged molecule and is moved by electric curren ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively charged molecule and is moved by electric curren ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we are separating molecules of DNA that we got from ...

Capillary electrophoresis is a very sensitive analytical technique. Sample components are separated within a fused silica capillary using one of several modes of electrophoresis. The technique can be ...

NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are intended for denaturing conditions only.NuPAGE Electrop ...

1. Prepare 20X TBE as:216 g Tris Base110 g Boric Acid80 mL 500 mM EDTA pH 8.0700 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Prepare Acrylamide solution as:6 % Acrylamide 60.0 g Acrylamide0.25 % Bi ...

Acrylamide Urea Gel (35 ml)10%15%40/2% acrylamide 10 ml 13.1 ml10X TBE 3.5 ml3.5 mlUrea 15 g15 gH20 10ml7.0 mlMicrowave ~10 seconds and stir until dissolvedDegas 1-2 minAdd 0.3 ml 10% ammonium persu ...

Native Acrylamide Gels (35 ml)3.5%5%6%8% 30/0.8% acrylamide 4.1 ml 5.8 ml7 ml9.3 ml 10X TBE 3.5 ml3.5 ml3.5 ml 3.5 mlH20 27.4 ml25.7 ml24.5 ml22.2 mlDegas 1-2 minAdd 0.25 ml 10% ammonium persulfateAd ...