常用设备/其他

1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution or a suspension of bacteria or bacteriophage.2. Typical diluions are 10-fold dilutions; each dilution is 1/ ...

1) Wash slides in Decon solution for 30 minutes. 2) Wash slides in running tap water for 30 minutes. 3) Wash slides in distilled water for 2 x 5 minutes. 4) Wash slides in 95% ethanol for 2 x 5 minute ...

Caution: Wear gloves and lab coat. Work with dichlorodimethylsilene in hood. Fill large dessicator with glassware. Put 1 ml dichlorodimethylsilene in a small beaker and put it in the dessicator. Pull ...

Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria fungi mycoplasma and cross contamination with other cell lines. All ...

Preventing surgical site infection in the operating room is the primary goal of the surgical team and all activities performed by the team support this goal. Some of these activities include patient r ...

You will need:- 12g Potassium Permanganate 6g Crushed paraformaldehyde 1) Set the hood so that it can vent outside. 2) Mix the two chemicals above together (in a jam/coffee jar so it can be thrown awa ...

Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers to procedures by w ...

Procurement100ml DEPC from Sigma (D5758) - �33/$292 (as of 2009-01) 100ml DEPC from MPI (150902) - �13 (as of 2009-01) DEPC-treatment of solutionsadd 0.05-0.1% (v/v) DEPC to solution/water (More DEP ...

Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not questioned and quickly become dogma. Furthermore it ...

RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA and tips for optimizing each of these methods. UV Sp ...

目的为动物来源性产品中150多种兽药残留的筛查提供一种有效的解决方案。前言兽药广泛的应用于治疗或预防动物疾病，但会带来肉、鱼、奶、蛋和蜂蜜等动物性产品中的痕量药物残留。由于对人体健康存在潜在的危害，食品链中药物残留的存在也引起了广泛的关注。确实，污染的动物产品可能导致过敏反应或由于细菌抵抗的发展导致临床治疗上间接的问题。为了保护消费者的健康并确保动物性产品的质量，在世界范围内设立了动物产品中药物允 ...

一、琼脂糖凝胶的特点天然琼脂（agar）是一种多聚糖，主要由琼脂糖（agarose，约占80％）及琼脂胶（agaropectin）组成。琼脂糖是由半乳糖及其衍生物构成的中性物质，不带电荷，而琼脂胶是一种含硫酸根和羧基的强酸性多糖，由于这些基团带有电荷，在电场作用下能产生较强的电渗现象，加之硫酸根可与某些蛋白质作用而影响电泳速度及分离效果。因此，目前多用琼脂糖为电泳支持物进行平板电泳，其优点 ...

Units1 mg = 10-3 g1 ug = 10-6 g1 ng = 10-9 g1 pg = 10-12 g 1 kb of double stranded DNA = 660 kD 1 kb of single stranded DNA = 330 kD 1 kb of single stranded RNA = 340 kD Average MW of a deoxynucleotid ...

Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-. This highly reactive molecule attacks o-position o ...

In the early eighties a series of papers were published by Gorm DANSCHER Aarhus Denmark to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallograp ...

细胞培养器材的选择：从大小来讲，细胞培养瓶约可分为约600ml，250ml，50ml和25ml的，一般都经过表面改性处理，适合细胞贴壁和生长。600ml 、300ml的多用于大规模培养时用（如单克隆细胞的培养等），50ml的多用于一般性细胞实验用（一般性的传代，保存细胞，为实验提供细胞等），25ml一般是用来复苏细胞或细胞较少时的培养，还有做原代细胞时也可以做多瓶而避免交叉污染。当然还有其他如圆形 ...

A few cell types are thin enough to be viewed directly in a microscope (algae protozoa blood tissue cultures) but most tissues (kidney liver brain) are too thick to allow light to be transmitted throu ...

Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. with a single colony. Use all 5 mL to inoculate a ...

Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4Cl 29.7 mM NaHCO3 25 µM Na2EDTA) 20.75g. NH4CL2.5g NaHCO30.093g Na2EDTA1L H2OHemocytometer Hema ...

1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid t ...