常用设备/其他

I. Useful Values 1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100000; in reality it is ~110000 but almost all tubulin labs use this convenient conversion relationship) 1 µm of a m ...

Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted). Make up the developer and the fixer and place in the darkroom ready for later (only if developing ...

WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendioxide a toxic gas when initially mixed.Laboratory S ...

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide. Decontamination of solutions 0.5mg/mlThis method reduc ...

The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics optics and mathematics by T. Svedberg and J.W. Williams i ...

Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the effect of gravity on particles (including macromolecu ...

Reaction volume 100 ul5 ul NADPH (100 mM)x ul cell extract so you get 50 % conversion0.2 uM C14-Testosteroneadd Tris-Citrate Buffer pH 6 to an endvolume of 100 ulmix C14-Testosterone and Citrate Buffe ...

Figure G.1 Electromagnetic Radiation Spectrum Figure G.2. Schematic light transmission Figure G.3. Use of the Spec 20 A spectrophotometer or colorimeter makes use of the transmission of light through ...

We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can ...

We regularly reuse our large 25x25 cm plating trays; initially however we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory ...

1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution or a suspension of bacteria or bacteriophage.2. Typical diluions are 10-fold dilutions; each dilution is 1/ ...

Caution: Wear gloves and lab coat. Work with dichlorodimethylsilene in hood. Fill large dessicator with glassware. Put 1 ml dichlorodimethylsilene in a small beaker and put it in the dessicator. Pull ...

UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/urea gels. The technique utilizes shortwave UV light (254 nm) and a fluor-coated TLC plate. We recommend UV shadowing ...

You will need:- 12g Potassium Permanganate 6g Crushed paraformaldehyde 1) Set the hood so that it can vent outside. 2) Mix the two chemicals above together (in a jam/coffee jar so it can be thrown awa ...

Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers to procedures by w ...

Procurement100ml DEPC from Sigma (D5758) - �33/$292 (as of 2009-01) 100ml DEPC from MPI (150902) - �13 (as of 2009-01) DEPC-treatment of solutionsadd 0.05-0.1% (v/v) DEPC to solution/water (More DEP ...

Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not questioned and quickly become dogma. Furthermore it ...

RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA and tips for optimizing each of these methods. UV Sp ...

除了某些细胞外的多肽激素和某些蛋白质与酶以外，对于细胞内或多细胞生物组织中的各种生物大分子的分离纯化，都需要事先将细胞和组织破碎，使生物大分子充分释放到溶液中，并不丢失生物活性。不同的生物体或同一生物体的不同部位的组织，其细胞破碎的难易不一，使用的方法也不相同，如动物脏器的细胞膜较脆弱，容易破碎，植物和微生物由于具有较坚固的纤维素、半纤维素组成的细胞壁，要采取专门的细胞破碎方法。1.机械破碎（1） ...

原理：这一方法基于考马斯亮蓝G-250有红蓝两种不同的形式。在一定浓度的乙醇及酸性条件下，可配成淡红色的溶液，当与蛋白质结合后，产生蓝色化合物，反应迅速而稳定。反应化合物在465－595nm处有最大的光吸收值，化合物颜色的深浅与蛋白浓度的高低成正比关系，因此可检测595nm的光吸收值的大小计算蛋白的含量。溶液：①Bradford储存液100ml95％乙醇200ml88％磷酸350mgServaG蓝 ...