常用设备/其他

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes. 2. Add 4 ml water centrifuge @ 2000 rpm in b ...

Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsAuthor: David Van Horn Greg BulajSource: Contributed by David Van Horn Dept. of Chemistry UC Berkeley Greg Bulaj Dept. of Biology Unive ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

T and B cell IsolationAuthor: Nanci E DonackiSource: Contributed by Nanci E DonackiAbstract: Isolate T and B cell from peripheral blood.Procedure Reagents Heparin - 1000 U/ml Ficoll-Hypaque PBS RPMI- ...

Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the donor. Disproportionate numbers of white cells (l ...

DNA Molecular Weight MarkersLambda DNA Hind III DigestFragmentBase Pairs123310294153655744361523226202775648125Lambda DNA EcoR I DigestFragmentBase Pairs1212262742135804456435487863530Lambda DNA Hind ...

Announcement Codon Usage Database is an extended WWW version of CUTG (Codon Usage Tabulated from GenBank). The frequency of codon use in each organism is made searchable through this World Wide Web si ...

Alphabetic List of Commercial Isoschizomers and Corresponding Fermentas Restriction EndonucleasesInformation on commercially available restriction endonucleases is from:Roberts R.J. and Macelis D. Nuc ...

Asymmetric sequences are indicated by *.Single letter code:R = G or A;Y = C or T;W = A or T;M = A or C;K = G or T;S = C or G;H = A C or T;V = A C or G;B = C G or T;D = A G or T;N = G A T or C.R ...

生物碱类（Alkaloids） 是存在于生物体（主要为植物）中的一类含氮的碱性有机化合物，大多数有复杂的环状结构，氮素多包含在环内，有显著的生物活性，是中草药中重要的有效成分之一。如黄连中的小檗碱（黄连素）、麻黄中的麻黄碱、萝芙术中的利血平、喜树中的喜树碱、长春花中的长春新碱等。 含生物碱的中草药很多，如三尖杉、麻黄、黄连、乌头、延胡索、粉防已、颠茄、洋金花、萝芙木、贝母、槟榔、百部等，分布于10 ...

腺病毒载体的优点：1. 宿主范围广 对人致病性低腺病毒载体系统可广泛用于人类及非人类蛋白的表达。腺病毒可感染一系列哺乳动物细胞，因此在大多哺乳动物细胞和组织中均可用来表达重组蛋白。特别需要指出的是：腺病毒具有嗜上皮细胞性，而人类的大多数的肿瘤就是上皮细胞来源的。另外，腺病毒的复制基因和致病基因均已相当清楚，在人群中早已流行（70-80%成人体内都有腺病毒的中和抗体存在）。人类感染野生型腺病毒后仅产 ...

Buffer Formula (required precision; 2 %)Always to try to prepare buffer solution at the temperature and concentration before planing to use during the experiment. Stock solutions are acceptable as lo ...

PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOHPBS (5x in 500 mls) 20.45 g NaCl 0.465 g KCl 10.142 g Na2HPOundefined7 H2O 0.545 g KH2PO4 pH 7.2Mounting Media 20mM Tris pH 8. ...

Sterile water bottles 200 ml 500 ml or 950 ml water.Autoclave.0.05 M CaCl2 (per 200 ml) CaCl2・2 H2O 1.5 gAdd 198 ml water.Autoclave.0.5 M CaCl2 (per 200 ml) CaCl2・2 H2O 15.0 gAdd 188 ml water.Autoclav ...

1. Simple Dilution (Dilution Factor Method)A simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid to achieve the de ...

AcO---- part one ---(AcO)2Ca 1; 1.5MAcOK 1; 2; 3; 4; 5; 6; 7; 8M(AcO)2Mg 1MAcONa 1; 2; 3; 4MpH of Ac K/Na solutionsAcONH4 1; 2; 3; 4MCO3-K2CO3 1; 2; 3; 4; 5; 6MKHCO3 1MNa2CO3 1; 2MNaHCO3 1M( ...

Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M 2.2MdNTP’s 100mMEDTA 0.5MEtBr 10mg/mlGelatin 2%Glucose 1; 1.5; 2MGuanidine HCl 1 ...

The following is a list of the solutions and chemicals required throughout the laboratory manual. It is organized alphabetically and individual exercises list the materials needed for that exercise. F ...

Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the effect of gravity on particles (including macromolecu ...