常用设备/其他

1、基本原理 在光学显微镜下无法看清小于0.2µm的细微结构，这些结构称为亚显微结构（submicroscopic structures）或超微结构（ultramicroscopic structures；ultrastructures）。要想看清这些结构，就必须选择波长更短的光源，以提高显微镜的分辨率。1932年Ruska发明了以电子束为光源的透射电子显微镜（transmission e ...

工欲善其事，必先利其器。对照一下，你的实验室还缺那些？ （1）超声波细胞破碎机 适用于5－100 g湿菌体超声破碎，实验室规模用。一般可选择直径10、15、20 mm的超声探头。推荐国产机器为宁波新芝。 （2）高压匀浆机 大规模生产用，对菌体量无限制。破菌效果好，但要注意冷却。 （3）高分散乳化机 均匀悬浮菌体用，比搅拌快，均匀性好，特别适合于大规模生产。另外，大规模生产时缓冲 ...

　　一、放射免疫分析的基本试剂　　（一）标准品　　标准品是放射免疫分析法定量的依据，由于以标准品的量用来表示被涮物质的量，故标准品与被测物质应当化学结构一致并具有相同免疫活性。标准品作为定量的基准。应要求高度纯化。标准品除含量应具有准确性外，还应具备稳定性，即在合理的贮存条件下保持原来的特性。　　按实验要求，将标准品用缓冲液配成含不同剂量的标准溶液，用于制作标准曲线。　　（二）标记物　　标记抗原应 ...

一、核酸及蛋白质常用数据 1．核苷三磷酸的物理常数化合物分子量λmax(pH7.0)1摩尔溶液（pH7.0）中λmax时的最大吸收值OD280/OD260ATP507259154000.15CTP48327190000.97GTP523253137000.66UTP484262100000.38dATP494259152000.15dCTP46727193000.98dGTP50725313700 ...

放射性核素数据1．放射性核素衰变表3H35S32P125I131I时间(年)剩余活性(%)时间(年)剩余活性(%)时间(年)剩余活性(%)时间(年)剩余活性(%)时间(年)剩余活性(%)194.5298.4195.3495.50.298.3289.3596.1290.8891.20.496.6384.41092.3386.51287.10.695.0479.81588.7482.51683.11. ...

Exposing gels and plates containing radioactive samples to X-ray film Although most people use the PhosphorImager for western blots kinase assays and methionine-labeled samples X-ray film remains the ...

CentrifugationThe single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics optics and mathematics by T. Svedberg and J ...

Rotor equilibration without counterbalanceContributed by Dr.A.GratchevThis is relatively obvious method which I found not used by most of the researchers. Usually the equilibration of 234689 and 10 tu ...

Radioactive DecayIsotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable however radioisotopes are unstable and disintegrate ...

I. Safety Procedures A. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by law to supply the user with pertinent information ...

Reaction volume 100 ul5 ul NADPH (100 mM)x ul cell extract so you get 50 % conversion0.2 uM C14-Testosteroneadd Tris-Citrate Buffer pH 6 to an endvolume of 100 ulmix C14-Testosterone and Citrate Buffe ...

With the aid of spectroscopy the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories. It includes absorption measurements in the ultravi ...

Characterization of dnaLEVEL I Materials DNA sampleSSC bufferUV spectrophotometer 3 and quartz cuvettes Procedure Dissolve a small quantity of your extracted DNA in 3.0 ml of 0.1X SSC. Turn on and bla ...

Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic so ...

Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be ...

BIURET PROTEIN ASSAYMATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard dilutions of BSA containing 1 2.5 5.0 7.5 and 10 mg/ml protein. Prepare s ...

Protein AssaysBelow is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range volume/amount of sample needed subjective comments on acc ...

Cell Isolation Theory Tissue TypesThis section summarizes the general characteristics of extracellular matrices associated with various types of tissue. Coupled with the descriptions of individual enz ...

1.1. Can the NCIS Kit be used on adults?The NCIS kit is specifically packaged for five separate tissue dissociations each containing up to twelve neonatal rat hearts. The NCIS protocol is available on ...

Cell Isolation Techniques Methods and MaterialsWorking With EnzymesAll of the enzymes Worthington offers for tissue dissociation applications are available as lyophilized powders for convenience vers ...