实验动物模型

Making library DNA from the DNA we send youThe library is distributed as 18 individual pools in the form of DNA. You will be sent about a microgram of each. Transform a suitable amount into E. coli ( ...

Replication timing by comparative hybridizationM. K. RaghuramanReference: Friedman K. L. M. K. Raghuraman W. L. Fangman and B. J. Brewer (1995) Analysis of the temporal program of replication initiati ...

Replication timing by density transfer M. K. Raghuraman 1. Grow cells at least 7 generations in0.1% (w/v) C-acetate or glucose 0.01% (w/v) N-ammonium sulfate Amino acids/supplements (as required added ...

Vectorette PCR of Yeast DNACarl Friddle1) Cut 1-3 µg of clean DNA overnight with 8-10U of blunt cutting enzyme in 20µl Most problems come from dirty uncut DNA. Phenol/glass bead/RNase prepared DNA wor ...

　1 甲醇酵母表达系统的特点　　1.1 宿主　　七十年代巴斯德毕赤酵母曾被用于生产单细胞蛋白（SCP），有很好的发酵基础，菌体密度可达100g/L干重。其生长培养液的组分包括无机盐、微量元素、生物素、氮源和碳源，廉价而无毒。它能在以甲醇为唯一碳源的培养基中快速生长，其中醇氧化酶AOX――甲醇代谢途径的关键酶可达细胞可溶性蛋白的30％。而在葡萄糖、甘油或乙醇作为碳源的培养细胞中则检测不到AOX。AO ...

问：如果诱饵蛋白对酵母细胞是有毒的，该怎么办？答：在有些情况下，在液体培养基中培养不好的菌珠可以在固体培养基上生长得很好。重悬克隆于1ml的SD/�Trp，接着将重悬液平铺于5 个100-mm的SD/�Trp平板。在30℃下温浴，直至平板上的克隆互相粘在一起。用5ml 0.5X YPDA刮下每块板上的克隆，并收集到一管中。接着就使用这个细胞重悬液进行正常的杂交反应。问：我的诱饵蛋白能直接激活报告基 ...

1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The list of such proteins needing further characterizati ...

DNA preparation from mouse tails General precautions to be followed during handling of any genomic DNA sample: Avoid Shearing of the DNA by limiting mixing to inversion and gentle shaking. Vortexing o ...

Blastocyst embryo transfer into pseudopregnant recipient female mouseBlastocyst transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluat ...

ES / MEF cell culture and electroporation of targeting constructDay 0 One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in a 37oC water bath. When the last bit of ice is melted ...

Always fill-in a form for each mouse you find/submit and make as many notes as possible about his behaviour health status or gross features. Always weigh the mouse. if mouse is found dead : Almost inv ...

You need to buy glass slides with frosted sandblasted ends (Fisher Scientific Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges ...

Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimen ...

Forms for submitting specimens to Pathology. Columbia University ICG onlyIn order to have full communication between the originator of the mouse and the pathologist you must fill a form with all the ...

Naming mouse strainsThere are internationally accepted rules to name transgenic and knock-out mice. See the following links: Mouse Nomenclature Rules and Guidelines These are suggestions about how to ...

How to keep track of your mice.Breeding mice can be very challenging particularly when you have to breed complex genotypes.You need a way to identify the mice permanently and a way to record birth gen ...

1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1-2h @ 37oC with occassional swirling. 3. Phenol extract GENTL ...

Animals are normally euthanized at the end of a study for the purpose of sample collection or post-mortem examination. Animals may be euthanized because they are experiencing pain or distress. Euth ...

RationaleThe use of mouse genetic models requires an efficient system of unique animal identifiers. The means that tissue must be obtained from each animal for genotyping and that each animal must be ...

Micro-CT检测肿瘤小鼠模型实验报告.pdf