实验动物模型

1-2 g fresh material freezer-dried ground with 0.2g sand (if necessary) and then homogenized with 10ml RNA extraction buffer (see below). Spin at 8000rpm 4oC for 10 minutes Remove the supernatants to ...

1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1-2h @ 37oC with occassional swirling. 3. Phenol extract GENTL ...

Animals are normally euthanized at the end of a study for the purpose of sample collection or post-mortem examination. Animals may be euthanized because they are experiencing pain or distress. Euth ...

General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has great potential for causing pain or distress to an ...

RationaleThe use of mouse genetic models requires an efficient system of unique animal identifiers. The means that tissue must be obtained from each animal for genotyping and that each animal must be ...

显微注射用BAC DNA的制备关于赛业（广州）生物科技 赛业（广州）生物科技有限公司 Cyagen Biosciences(Guangzhou) Inc.是美国 Cyagen Biosciences Inc. 旗下中国区子公司，专业从事干细胞、分子生物学及转基因模式动物的前沿技术研发。是华南地区唯一一家专门从事干细胞和转基因小鼠产业化的高新技术企业，在科研、管理、场地、设备、材料、及市场 ...

显微注射用质粒DNA的制备关于赛业（广州）生物科技 赛业（广州）生物科技有限公司 Cyagen Biosciences(Guangzhou) Inc.是美国 Cyagen Biosciences Inc. 旗下中国区子公司，专业从事干细胞、分子生物学及转基因模式动物的前沿技术研发。是华南地区唯一一家专门从事干细胞和转基因小鼠产业化的高新技术企业，在科研、管理、场地、设备、材料、及市场化工作 ...

day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C shaking. 3. Prepare: ~ 3.5 L sterile H2O chilled to ...

This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993) Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are made that any of the steps are necessary or ideal; th ...

1. Immerse Arabidopsis seeds in 10% Household Bleach for 20 min. 2. Rinse the seeds twice with 500 ml of sterile ddH2O and allow them to dry overnight in a laminar flow hood. 3. Push the resulting cru ...

Using this procedure it is possible to follow the development of pollen mother cells through to mature pollen. Sterile males were made using irradiation mutagenesis of Landsberg erecta seed. Buds harv ...

Materials: fixative dependent on antigen (common: 4% formaldehyde or 100% MeOH) post-fixative dependent on antigen (usually MeOH) PBS 1X PBS PBST PBS + detergent (0.05 to 0.5 % Tween or Triton) DAPI ( ...

Preparation of the probe. Probes are prepared as Digoxigenin labelled RNA . The labelling mix as well as all antibodies are purchased from Boehringer All conditions and solutions should be totally RN ...

There are two kinds of contaminants on worm plates:1. Fungi: these contaminants can come from the plates or bacteria so it is best to leave plates out after seeding for a couple of days to make sure n ...

Stockkeeping1. MechanicsMost stocks can be successfully cultured by periodic mass transfer of adults to fresh food. Bottles or vials are tapped on the pounding pad to shake flies away from the plug th ...

Equipment flies frozen in 250 ml centrifuge bottlessieves: 106 355 600 and 850 mmpaintbrushcollection tubesfunneldry icejacket hat and gloves! Preparation 1. Freeze flies in 250 ml centrifuge bottle(s ...

Gloor et al. 1993 Genetics 135:81-95We have developed a simple method for the rapid and reproducible isolation of DNA from single flies for amplification by the polymerase chain reaction (PCR) (Saiki ...

.1 RNA Probe Preparation (see Note 1) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates.2. RNAse free water.3. T7 T3 or SP6 RNA Polymerase (Fermentas Life Sciences Burling ...

ProcedureTransfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract 2% peptone 2% dextrose) into a microcentrifuge tube. Pellet cells by centrifugation at 20000 × ...

1.Inoculate a 5 ml culture with a single colony from a YAC-containing strain of yeast and grow until saturated. Determine the number of yeast cells per milliliter. The cell count should be roughly 1 x ...