RNA原位杂交

mRNA PurificationI. Prepare oligo-dT celluloseuse 40 mg oligo-dT cellulose / 1 mg total RNA swell oligo dT-cellulose in elution buffer wash oligo dT-cellulose 4 x with elution buffer (30 sec. full sp ...

Nucleotide Composition of RNALEVEL II Materials RNA sample1 N and 0.1 N HClBoiling water bathWhatman #1 filter paper (for chromatography)Chromatography tank20 µl micropipetteAcetic acid: butanol: wate ...

mRNA PurificationI. Prepare oligo-dT celluloseuse 40 mg oligo-dT cellulose / 1 mg total RNA swell oligo dT-cellulose in elution buffer wash oligo dT-cellulose 4 x with elution buffer (30 sec. full sp ...

Nucleotide Composition of RNALEVEL II Materials RNA sample1 N and 0.1 N HClBoiling water bathWhatman #1 filter paper (for chromatography)Chromatography tank20 µl micropipetteAcetic acid: butanol: wate ...

PolyATtract® mRNA Isolation SystemsThe PolyATtract® mRNA Isolation Systems utilize Promega's MagneSphere® technology to eliminate the need for oligo(dT) cellulose and its associated problems. With tot ...

When isolating RNA it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also beneficial to know rough expression levels for rar ...

When isolating RNA it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also beneficial to know rough expression levels for rar ...

Spectrophotometric Analysis of rRNALEVEL I Materials RNAUV spectrophotometer and cuvettesAlkaline distilled water Procedure Dissolve 10 mg of commercial RNA in 250 ml. of slightly alkaline distilled w ...

Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENT *** Materials Rat liver (fasted rat)Liquid nitrogenp-Amino-salicic acidPhenol mixtureHomogeniz ...

Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENT *** Materials Rat liver (fasted rat)Liquid nitrogenp-Amino-salicic acidPhenol mixtureHomogeniz ...

N.B: Care must be taken when handling solutions containing high concentrations of guanidine salts due to its chaotropic nature. As with other procedures involving RNA gloves should be worn at all tim ...

DROSOPHILA RNA PREP(Goodman Lab) Stock Solutions 3M NaOAc pH 5.2 with HAc (MW=82) 6M Guanidine Hydrochloride in 0.1M NaOAc pH 5.2 (MWGuHCl=95.54) 5.7M Cesium Chloride in 0.1M NaOAc pH 5.2 (MWCsCl=168. ...

DROSOPHILA RNA PREP(Goodman Lab) Stock Solutions 3M NaOAc pH 5.2 with HAc (MW=82) 6M Guanidine Hydrochloride in 0.1M NaOAc pH 5.2 (MWGuHCl=95.54) 5.7M Cesium Chloride in 0.1M NaOAc pH 5.2 (MWCsCl=168. ...

LiCl RNA Preparation (Ambros Lab)1) Freeze worms by dropping into liquid nitrogen 2) Grind to a fine powder in a mortar that was precooled with liquid nitrogen 3) Transfer powder to a Falcon tube 4) A ...

Rules of siRNA design for RNA interference (RNAi)Source: Protocol OnlineAbstract: Summarize both Tom Tuschl's motif siRNA design rules and siRNA rational design.General GuidelinessiRNA targeted sequen ...

Isolation of RNA from Difficult TissuesThe often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and ...

Isolation of RNA from Difficult TissuesThe often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and ...

One of the most problematic steps in RNA isolation is the first step - thorough lysis of the tissue or cell sample in a denaturant solution that inhibits RNA degradation by RNase. While it is possible ...