RNA原位杂交

Northern BlotI. Electrophoresisclean gel box with NaOH and/or SDS 2 hours to overnight rinse with water prepare Agarose gel solution 1 x MOPS H2O to 95 % of endvolume microwave until completely diss ...

Northern BlotI. Electrophoresisclean gel box with NaOH and/or SDS 2 hours to overnight rinse with water prepare Agarose gel solution 1 x MOPS H2O to 95 % of endvolume microwave until completely diss ...

NorthernsStuff you need:10X running buffer:50 mM NaOAc0.2M MOPS pH 7.010 mM EDTAfilter store in dark at RT pH to 7.0Formaldehyde gel:0.9% agarose in 1X running buffer and 1ml formaldehyde per 20 ml ge ...

Orcinol Determination of RNALEVEL I Materials RNAAlkaline distilled waterAcid-orcinol reagentBoiling water bathSpectrophotometer and cuvettes Procedure Prepare a series of serially diluted RNA standar ...

Orcinol Determination of RNALEVEL I Materials RNAAlkaline distilled waterAcid-orcinol reagentBoiling water bathSpectrophotometer and cuvettes Procedure Prepare a series of serially diluted RNA standar ...

Preparation of Poly A+ RNA and Northern AnalysisProcedure (Pharmacia #27-9255-01) Use RNase-free tips and reagents and wear gloves1) Remove organs from six C57Bl mice (do cervical dislocation immediat ...

Pre-hyb/hyb for Radioactive ProbesStuff you need:50X Denhardt's reagent:1% Ficoll1% Polyvinylpyrrolidone1% Bovine Serum AlbuminPrehyb/hyb solution: 5X SSC50% formamide100 µg/ml SS DNA (heat denatured ...

Primer Extension analysis of total yeast RNA 1. Mix the following in a 1.5 ml eppendorf tube:a. 20-80 micrograms yeast RNA dissolved in H20 (the amount will depend on the level of expression for the g ...

3'RACE PCR3' Rapid Amplification of cDNA Ends (RACE) PCR This technique is used to obtain the 3'end of a cDNA it requires some sequence information internal to the mRNA under study. The sequence infor ...

点击浏览该文件RACE系列栏目：RACE试剂盒PCR/RT-PCR/qPCR RACE方法相关产品:RACE试剂盒 SAGE 试剂盒 核酸酶 聚合酶 反转录酶限制性内切酶 PCR克隆试剂盒 常用克隆载体克隆试剂盒 电泳设备 紫外设备 普通PCR仪 定量PCR仪 PCR/RT-PCR/qPCR试剂PCR引物 PCR试剂PCR对照特异性PCR试剂盒PCR克隆试剂盒RNA RNase检测/去除 RT-P ...

The "Neverfail" Northern Blot HybridizationContributed by James McCaughern-Carucci Bristol-Myers Squibb 10/26/97 Prehybridization/Hybridization Solution0.1% SDS50% Formamide5x SSC50 mM NaPO4 pH 6.80.1 ...

In vitro transcription with yeast nuclear extractSteve HahnLast Modified Fri Apr 25 2003Wear gloves throughout use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipe ...

1.0 g plant tissue:grind tissue with pestle and mortar in liquid nitrogen transfer to a 50 ml falcon tube containing 10 ml of sol D add 1 ml 2 M NaOAc pH 4.1 add 10 ml phenol add 2 ml chloroform/isoam ...

1. Collected animals can be stored as frozen pellets at -70 degrees. Alternatively freshly collected animals can be quick frozen in liquid nitrogen. Prechill a mortar and pestle with liquid nitrogen. ...

Introduction:C. elegans RNA preps that have been widely used previously involved fairly slow lysis of the worms potentially leading to degradation of the RNA. In addition they failed to separate RNA f ...

Primer Extension System - AMV Reverse TranscriptaseThe Primer Extension System is used to determine the location and level of a target 5´end of RNA. The System uses AMV Reverse Transcriptase (Cat.# M ...

A frequent cause of concern among investigators performing quantitative RT-PCR is false positives caused by genomic DNA contamination of RNA preparations. Because PCR is such a sensitive technique a s ...

How to prepare Molecular Biology grade glycogenAuthor: Roberto SalviSource: Contributed by Roberto SalviAbstract: Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitat ...

Concentrating poly(A)+ mRNASource: Protocol OnlineAbstract: Simple precipitation method of concentrating poly(A)+ mRNA.ProcedureAdd 1/10 volume 3 M NaAc pH 5.2 and 2.5 volumes 100% ethanol. Mix and in ...

Purification of poly(A)+ RNA from total RNA0. (Optional)Before using Oligotex-dT30 I recommend to wash Oligotex-dT30 to remove fine particle of latex. To wash the latex transfer appropriate amount of ...