RNA原位杂交

详细的使用说明请见下面的PDF文档！B5印刷版 siRNA产品使用说明.pdf订购产品请详细阅读和填写下面的合同和信息卡发到：sales-cd@ribobio.com！Ribobio 2010年购销合同.docsiRNA 订购信息卡.xlssiRNA套装 订购信息卡.xls ...

锐博生物是国内最专业的miRNA研究服务公司，可提供miRNA研究的全套相关产品和服务！详细的实验操作请见下面的PDF文档！B5印刷版 miRNA产品使用说明.pdf需要订购相关产品请填好下面的合同和订购信息卡发到：sales-cd@ribobio.com！Ribobio 2010年购销合同.docmiRNA 订购信息卡.xls广州锐博real-time PCR检测委托服务单.doc3 ...

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DNA probes are prepared using a modification of the method of Feinberg and Vogelstein (1983). You will need: Nuclease-free BSA-dCTP (Amersham or DuPont)Klenow DNA Polymerase (New England Biolabs or Ph ...

Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344)OverviewThere are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass f ...

Gel Setup Using Protean II systemClean materials with 10% SDS and rinse thoroughly with ddH20 Set up gel apparatus as per normal (use thick spacers) 1.5mM Make up 15% Denaturing Gel Northern Gel # of ...

MicroRNAs (miRNAs) are an important class of small RNAs that regulate protein translation (see Getting Started with MicroRNA Investigation). To date 230 mammalian miRNAs have been identified and catal ...

This protocol is for four time-points in 12-well plates. Scale up or down as needed.Day 1: Plate cells:Split one or more 10-cm plate(s) with 80-100% confluent HeLa Tet-off cells to a final volume of ...

The primer extension re action is used to determine the start site(s) of RNA transcription for a particular gene. One uses a radiolabelled primer that is complementary to a region towards the 5'' end ...

RNA isolationPrimer LabelingTo 0.5pmole primer (0.5µl 10pmoles/µl) add 1µl 10x T4 polynucleotide kinase buffer from Roche kit. Add 2.5µl 32P-γ-labelled dATP (5µl if using 32P-γ-labelled dATP past it'' ...

PurposeTo convert RNA into cDNA. You will use a protein called Reverse Transcriptase which is a polymerase that synthesizes DNA from RNA. The reaction requires primers which can be either oligodT (an ...

ATTENTION: This is expensive. One reaction as described here is ca. 6 Euro!Check the quality of your RNA by gel to be sure it is not degraded.Prepare the annealing mix: RNA50 ng/µl1 µgrandom hexamers ...

Reverse transcription is the reverse transcriptase-mediated synthesis of single-stranded DNA (complementary DNA or cDNA) using single-stranded RNA as template; the cDNA product and the template RNA ha ...

4l RT buffer9l H2O (RNA to be included in this vol but added last)2l 0.1 M DTT1l oligo dT primer2l 10mM dNTP''s1l RNasin1l Reverse Transcriptaseincubate for 1hr at 37C then heat inactivate at 70C for ...

We suggest the following procedure for total RNA isolation based on years of experience working with complicated samples and small amounts of starting material (Matz 2002). The procedure is suitable f ...

This protocol was modified from the following referece: Bar-Peled M. A.S. Conceicao L. Frigerio and N.V. Raikhel. 1995. Expression and regulation of aERD2 a gene encoding the KDEL receptor homolog in ...

Gene silencing by RNA interference is being used routinely to study gene function in cultured mammalian cells. While this approach has been extremely powerful it does not allow a critical evaluation o ...

In vivo Purity Stealth RNAi™ siRNA and BLOCK-iT™ siRNA duplexes are specifically formulated for use in animals. Resuspend the RNA duplex in UltraPure DNase/RNase-free distilled water or appropriate DN ...

OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse Refseq transcript. The algorithm applies a set of r ...

Day 1: Seed cells - should reach approximately 20% confluency the next day (e.g. use 150 μ l (from confluent p10 taken up into 10 ml) per well. 2 ml DMEM per well). It is important that the cells are ...