RNA原位杂交

We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Designed dsRNA). It is also possible to produce dsRNA u ...

6-Well Plates Bathing Prepare dsRNA suspended in water.We use ~500 bp dsRNA. Add ~10-30 µg dsRNA to wells of 6-well tissue culture plate.We use 0.1-0.3 µg in 384-well plates for 25-50 nM final concent ...

Injection DNA Preparation1) Culture 400 mls of bacteria harboring transposon overnight at 37oC. 2) Harvest cells at 7000xg for 5 min. (pellet can be frozen at -80oC for several weeks) 3) Resuspend pel ...

• Isolate total RNA and native protein from the same sample • Enrich for small RNAs ( • Ideal for assessing siRNA or miRNA expression and processing Small interfering RNAs (siRNAs) and microRNAs (miRN ...

Generate siRNA Populations with Dicer The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Id ...

Generate siRNA Populations with Dicer The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Id ...

The Easiest Route to Guaranteed Silencing Increasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induce ...

The Easiest Route to Guaranteed Silencing Increasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induce ...

MicroRNA and siRNA Cloning Protocol 点击浏览该文件SiRNA产品介绍:siRNA库：超过10000种基因的SiRNA库，拿来就用！siRNA技术相关试剂siRNA | 反义寡核苷酸 | RNAi Oligo合成 | RNAi定量 | RNAi文库 | miRNA分离 | miRNA分析 | siRNA酶 | siRNA标记 | siRNA纯化 | siRNA合成 ...

S1 analysis of yeast mRNA using oligonucleotide probes Steve Hahn. last modified Sat Oct 17 1998Mix the following in an 0.5 ml eppendorf tube:10-20 micrograms total yeast RNA10 microliters hybridizat ...

How to Maintain an RNase-free Lab Let's admit it everyone who has worked with RNA has at some point been affected by RNase contamination. Sources of contaminating RNase include procedures that use RNa ...

How to Maintain an RNase-free Lab Let's admit it everyone who has worked with RNA has at some point been affected by RNase contamination. Sources of contaminating RNase include procedures that use RNa ...

Decontamination and inhibition of ribonucleases It is common knowledge in most laboratories that RNA is more susceptible to degradation than DNA. Unlike Deoxyribonucleases (DNases) which require metal ...

Decontamination and inhibition of ribonucleases It is common knowledge in most laboratories that RNA is more susceptible to degradation than DNA. Unlike Deoxyribonucleases (DNases) which require metal ...

Add 0.1% diethylpyrocarbonate (DEPC) to water mix overnight and then autoclave 20 min to destroy DEPC by causing hydrolysis of DEPC.Notes:DEPC is highly toxic. ...

Solublization of RNA in Formamidecontributed by James McCaughern-Carucci Yale University Resuspending RNA in Formamide (as reported by Chomczynski et al. Nucleic Acids Research) has several benefits o ...

Phenol ExtractionOne of the most common molecular biology lab procedures phenol extraction removes proteins from nucleic acid samples during isolation and purifies nucleic acids after enzymatic reacti ...

A Detailed Procedure for RNase Activity Staining on SDS-PAGE 1. Remove glass plates that have been stored soaking in 3% hydrogen peroxide and dry thoroughly with Kimwipes. 2. Assemble plates and se ...

To the average molecular biologist working with RNA preventing detecting and eliminating nuclease contamination particularly ribonuclease (RNase) contamination is a constant and somewhat annoying chal ...