cDNA合成技术

First strand of cDNA synthsis 1.Add 2 μl of Not I primer-adapter to a sterile 1.5 ml RNAase free tube add 4.5 μl mRNA of molt4 cells (~5.2 ug) add 0.5 μl DEPC-H2O. 2. Heat the mixture at 70℃ ...

Promega公司的RibocloneR M-MLV(H- ) cDNA合成系统采用M-MLV反转录酶的RNase H缺失突变株取代AMV反转录酶，使合成的cDNA更长。该系统的第一链合成使用M-MLV反转录酶，cDNA第二链合成采用置换合成法，采用RNaseH和DNA聚合酶Ⅰ进行置换合成，最后用T4 DNA聚合酶切去单链末端，方法简便易行。该系统试剂包括： 20μg 特异性引物 200& ...

The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (Chromosoma 98 (1989) p81) but adapted to allow the screening of 96 RNA probes on whole-mount Drosophi ...

Prepare in a sterile tube: template RNA: total RNA 0.1-5µg or poly(A)+ mRNA 10ng-0.5µg or specific RNA 0.01pg-0.5µg primer: oligo(dT)18 0.5µg or ...

The Queensland Institute for Medical Research (QIMR) lab's method for automated sequencing for expressed sequence tags from Schistosoma japonicum and/or S. mansoni cDNA libraries constructed in the di ...