DNA蛋白相互作用

摘要 Trafficking of AMPA receptors (AMPARs) is regulated by specific interactions of the subunitintracellular C-terminal domains (CTDs) withother proteins but the mechanisms involvedin ...

摘要 mRNA decapping is a common step shared between twoimportant mRNA decay pathways in yeast Saccharomycescerevisiae. To investigate how mRNAs are decapped we havedeveloped an assay ...

摘要 In both prokaryotes and eukaryotes nonsense mutations in a gene can enhance the decay rate or reduce the abundance of the mRNA transcribed from that gene and we call this process nonsense-m ...

摘要In eukaryotic cells premature termination of translation at nonsense codons has been implicated as the cause of a variety of posttranscriptional events including rapid mRNA decay in the cytoplasm or ...

In both prokaryotes and eukaryotes nonsense mutations in a gene can enhance the decay rate of the mRNA transcribed from that gene a phenomenon described as nonsense-mediated mRNA decay. In yeast the ...

很多研究者因样品DNA很脏，转而求助于我们。问题样品主要集中在土壤、粪便和血液。样品富含腐植酸、多糖类、亚铁血红素和色素等，与DNA交联严重抑制下游PCR扩增等酶实验和测序。MO BIO拥有一项专利抑制因子去除技术®（IRT），应用在多款试剂盒上能高效去除抑制因子却极少影响DNA得率。这项技术真的有用吗？负责任的研究者需要看到实验数据。最近我们接到下面的一个问题：Q：你们有使用与不使用抑制 ...

短串联重复序列（short tandem repeat，STR），又称为微卫星DNA ，重复单位为2-6bp，重复次数10~60多次，在DNA复制过程中的滑动、复制、修复过程中滑动链与互补链的碱基错配，从而导致一个或几个重复单位的缺失或插入，形成STR的多态性，主要应用于遗传连锁图谱分析、家系鉴定、身份认证 ...

SNP(Single Nucleotide Polymorphisms)，即单核苷酸多态性，是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性，它是人类可遗传的变异中最常见的一种，并作为第三代遗传标志。人体许多表型差异、对药物或疾病的易感性等等都可能与SNP有关，因此被广泛用于群体遗传学研究和疾病相关基因研究。目前，SNP检测技术已经很成熟，主要有以下几种检测方法：TaqMan探针法、H ...

A. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (1718) followed b ...

Objective:Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis seque ...

We store our all of our plasmids as bacterial host stocks at -80 stored in 7% DMSO. Such stocks are very long-lived giving robust bacterial growth even after 10-15 years. However occasionally we hav ...

1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml m ...

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Jian Yu ( Email: jianyu@jhmi.edu)Last edited on May 1 2000OverviewThis protocol was developed in Kinzler/Vogelstein laboratories to isolate cDNA fragments corresponding to novel SAGE tags that do not ...

Map-Based Cloning of KAM2

Alkaline lysis miniprep

Note

IntroductionExtraction and purification of plasmid DNA from E.coli can be performed on a small scalewhere DNA is extracted from just 1-2 ml of bacterial culture (mini-prep) or from much larger volumes ...