基因组学

Intracytoplasmic Spermatocyte Injection (ICSI) The number of infertile couples has increased in recent years and is now estimated at 15�20% of all couples worldwide. Since its introduction into repro ...

Bacterial beta-Galactosidase Histochemisty BibleThis is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately I can't attribute ...

Lac-Z Detection in Tail Biopsies2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X ...

Lac-Z Detection in Tail Biopsies2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X ...

Transgenic Animals The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in th ...

Transgenic Animals The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in th ...

Methylated CpG island AmplificationProtocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI XmaIT4 DNA ligaseTaq DNA polymerase10X PCR reaction buffer:670mM Tris-HCl pH 8.840mM M ...

Methylated CpG island AmplificationProtocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI XmaIT4 DNA ligaseTaq DNA polymerase10X PCR reaction buffer:670mM Tris-HCl pH 8.840mM M ...

The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive research of methylation processes and it's influe ...

Frikke-Schmidt R Nordestgaard BG Jensen GB Tybjaerg-Hansen A. Department of Clinical Biochemistry Rigshospitalet Copenhagen University Hospital Copenhagen Denmark. Homozygosity for mutations in ABC tr ...

刘光清　刘在新　谢庆阁(中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室兰州730046)摘　要: 　反向遗传技术是一种新兴的分子生物学技术 已广泛应用于生命科学研究的各个领域。综述反向遗传技术研究进展并讨论该技术在口蹄疫病毒研究中的应用。关键词: 　反向遗传学　反向遗传技术　全长cDNA 　口蹄疫病毒Reverse Genetic Technique and Its Applicati ...

Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome and your sample genome. NimbleGen offers two high-d ...

Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome and your sample genome. NimbleGen offers two high-d ...

图位克隆(Ｍａｐ-ｂａｓｅｄｃｌｏｎｉｎｇ)又称定位克隆(ｐｏｓｉｔｉｏｎａｌｃｌｏｎｉｎｇ)1986年首先由剑桥大学的Ａｌａｎｃｏｕｌｓｏｎ提出用该方法分离基因是根据目的基因在染色体上的位置进行的无需预先知道基因的ＤＮＡ顺序也无需预先知道其表达产物的有关信息但应有以下两方面的基本情况:一是有一个根据目的基因的有无建立起来的遗传分离群体如Ｆ、ＤＨ、ＢＣ、ＲＩ等。二是开展以下几项工作:1)首先找到 ...

Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute Institute of Genetics and Biophysics CNR 80131 Naples ItalyExcerpted ...

生物在线评：这两份protocol都非常完整，细致，将ChIP实验中许多细节都进行了描述，是拿来就用行的。但各有千秋！后者更为细致，甚至精细到哪一天完成什么实验的地步。ChIP_Protocol5-29-07.pdfFarnhamLabChIP Protocol.pdf ...

生物在线评：这两份protocol都非常完整，细致，将ChIP实验中许多细节都进行了描述，是拿来就用行的。但各有千秋！后者更为细致，甚至精细到哪一天完成什么实验的地步。ChIP_Protocol5-29-07.pdfFarnhamLabChIP Protocol.pdf ...

同源重组（Homologus Recombination) 是指发生在姐妹染色单体（sister chromatin) 之间或同一染色体上含有同源序列的DNA分子之间或分子之内的重新组合。同源重组需要一系列的蛋白质催化，如原核生物细胞内的RecA、RecBCD、RecF、RecO、RecR等；以及真核生物细胞内的Rad51、Mre11-Rad50等等。在人类细胞中，同源重组由Rad51介导，在有A ...

TILLING-高通量点突变筛选方法 反向遗传学方法是功能基因组研究的重要方法。为了克服传统的基因敲除方法的局限性，TILLING（Targeting Induced Local Lesions In Genomes）技术通过化学诱变的方法产生的大量点突变个体，可以对部分功能缺失的基因及基因分型中亚致死等位基因进行研究。目前，TILLING技术已被大量应用于拟南芥、果蝇、玉米、斑马鱼、苜蓿 ...

Zuzana Jasencakova and Anja GrothBRIC (Biotech Research and Innovation Centre)University of CopenhagenOle Maaløes Vej 5DK-2200 Copenhagen N DenmarkIntroductionHere we describe a strategy for isolation ...