基因组学

PLoS Genet. 2009 March; 5(3): e1000433. Published online 2009 March 20. doi: 10.1371/journal.pgen.1000433A Genome-Wide Association Study Confirms VKORC1 CYP2C9 and CYP4F2 as Principal Genetic Determ ...

Genome mapping and molecular breeding of tomato.--2007.pdf(1.5 MB 下载次数: 0 售价: 金钱 10 )摘要： The cultivated tomato Lycopersicon esculentum is the second most consumed vegetable worldwide and a we ...

从DNA水平上寻找确诊遗传病的指标或探讨遗传病和肿瘤的病因等方面，已取得很大成绩，这对产前诊断，早期确诊和突变基因携带者的检出等都有重要意义。所用的方法大体有以下几种。　　一、分离基因进行结构分析　　利用DNA离体转化，制备探针，制备基因文库进行筛检，最后鉴定载体中插入DNA片段的特性等一系列技术，可以设法分离出目的基因或某一特定的DNA顺序。然后通过DNA核苷酸顺序分析可以弄清楚某些疾病的病因。 ...

第七章 RFLP和RAPD技术第一节 概 述　　DNA分子水平上的多态性检测技术是进行基因组研究的基础。RFLP(Restriction Fragment Length Polymorphism限制片段长度多态性)已被广泛用于基因组遗传图谱构建、基因定位以及生物进化和分类的研究。RFLP是根据不同品种（个体）基因组的限制性内切酶的酶切位点碱基发生突变，或酶切位点之间发生了碱基的插入、缺失，导致酶切 ...

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Clonality - X Chromosome Inactivation AssayThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the fo ...

Clonality - X Chromosome Inactivation AssayThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the fo ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

PCR Protocol10X PCR Buffer1.0MgCl2 (2.0mM)0.8µldNTPS miundefined0.8µlPrimer-F (µg/µl)0.1µlPrimer-R (µg/µl)0.1µlTaq (5U)0.1µl32P dCTP (10µci/µl)0.1µlddH2O4.5µlDMSO (100%)0.5µlSUBTOTAL8.0µlTemplate DNA (50-10 ...

This web site was designed to provide detailed protocols for genetic footprinting a technique for high-resolution mapping of the functional organization of cloned genes (Singh Crowley and Brown (1997) ...

Materials:Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media). Col ...

Materials:Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media). Col ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ob ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ob ...

DIFFERENTIAL DISPLAYComparible RNAs were isolated from different treatments using my favorite method or any other methods you like and dissolved in RNA water. DNase treatment of 20ug total RNA 37oC 30 ...

Differential Display of Cotton TranscriptsSong P. Yamamoto E. and Allen R. (1995) Plant Mol. Biol. Rep. 13:174-181Plant MaterialsCotton ovules (Gossypium hirsutum cv. Coker 312) were collected 8 15 an ...

Chromatin IP MethodDay 11. Start 5 ml o/n cultures of strains to be testedDay 21. Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200 rpm. Th ...

Human chromosome dimensionsChromosome # Relative length Centromere index 1 9.08 48.0 2 8.45 38.1 3 7.06 45.9 4 6.55 27.6 5 6.13 27.4 6 5.84 37.7 7 5.28 37.3 X 5.80 36.9 8 4.96 35.9 9 4.83 33.3 10 4.68 ...

The differential display protocol described here is based on the principle described by: Liang P. and A.B. Pardee : Differential display of eukaryotic messenger RNA by means of the polymerase chain re ...

All living organisms have thousands to tens of thousands of unique genes encoded in their genome of which only a small fraction perhaps 15% are expressed in any individual cell. Therefore it is the te ...