基因组学

For quantitating RNA levels from either polyA+ selected RNA or crude RNA preps. Solutions 10X Hybridization Buffer 0.4 M PIPES 12.1 g PIPES (Sigma #P6757) 4.0 M NaCl 23.4 g NaCl 10 mM EDTA 2.0 ml 500 mM EDTA pH to 6.7 and bring up to 100 ml with DEPC treated Q, autoclave 1X Hybridization Mixture 8 ml formamide 1 ml DEPC treated Q 1 ml 10X Hybridization Buffer 2X RNase Buffer 20 mM Tris 7.5 2 ml 1M Tris 7.5 10 mM EDTA 2 ml 500 mM EDTA 8.0 0.6 M NaCl 12 ml 5 M NaCl up to 100 ml DEPC treated Q, aut

Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it? thanks a million for any suggestions! -kiwi- Kiw ...

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Publisher: Bios Scientific Publishers Ltd Number Of Pages: 608 Publication Date: 2002-06-15 Sales Rank: 4476658 ISBN / ASIN: 1859962289 EAN: 9781859962282 Binding: Hardcover Manufacturer: Bios Scienti ...

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DNaseI Footprintint Solutions 10X Binding Buffer 200 mM Tris 8.0 200 m l 1M Tris pH 8.0 500 mM NaCl 100 m l 5M NaCl 10 mM EDTA 20 m l 0.5 M EDTA pH 8.0 680 m l Q store at room temperature DNaseI Dilut ...

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FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of: 15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up ...

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An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average every DNA molecule is c ...

This technique can be used to isolate overlapping DNA fragments starting with a previously cloned DNA fragment that maps near a gene of interest (dark red). The walk is continued until a clone containing the desired gene is identified. In this example, the chromosomal DNA fragments are cloned in λ pha ...

Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been utilized by the NCI Prostate Cancer Working Group see the section on Cell Lines.DNA RNAProteomicDNA sequence copy number changes throughout the genome in a single ...

点击浏览该文件Footprinting Analysis

Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome and your sample genome. NimbleGen offers two high-d ...