DNA提取与纯化

Sample Preparation Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brus ...

简介 优点：操作简单方便 缺陷：不能像EMSA或footprinting一样区分不同类型复合物 原理 双链 DNA 能够通过硝化纤维膜 (NC)，但是 DNA-protein 复合物不能通过NC filter. 通过定量分析留在滤膜上的复合物，可以判断二者的结合常数。 基本方法 Lable DNA Mix DNA and protein to form complex Pass th ...

Qiagen公司的琼脂糖凝胶回收试剂盒：MinElute Gel Extraction Kit 品牌：Qiagen回收范围：70bp-4kb价格：（50包装1485，约29元/反应） 特点： 洗涤体积只要10μl，回收产物浓度高，方便后继实验 使用方便快捷（wash-and-spin) 高回收率 回收产物纯度高，可用于同位素或荧光测序、芯片分析、连 ...

Materials: 3 M sodium acetate pH 5.2 or 5 M ammonium acetate DNA 100% ethanol Measure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium a ...

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CLONE ISOLATION PREP - PROTOCOL Before you begin isolating DNA from the cultures: --GENTLENESS is key through step 10! --Try to do prep steps 1-8 at the side of the centrifuge --Make Solution III and ...

Pellet 1.5 ml of an overnight culture at 12000 rpm in Eppendorf centrifuge at RT for 3 min. Resuspend bacterial pellet in 350 µl of STET buffer. Add 25 µl of freshly prepared solution of ...

This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples. Materials 3M Sodium Acetate buffer pH 5.2 (store at 4 ℃). Cold 100% Et ...

Molecularpourous membrane tubing is used for desalting protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see ge ...

Promega Wizard SV Gel and PCR Clean-up System 品牌：Promega 回收范围：100bp-10kb 价格：（50次包装的试剂盒报价为562元，11元/次） Promega的产品在进口品牌中一向以价格可亲而著称。这个胶回收试剂盒也不例外。并且这个试剂盒的性能参数好得令人刮目相看！首先是100bp—10kb的回收率高达 ...

1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin or 1mg/5ml. Optional: Use a 15ml conical tube with a loosened cap and a piece of tape to hold it in place. Shake at 2 ...

Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this ...

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Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases ...

Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie ...

Used to removed unincorporated nucleotides from labelling reactions. Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 m ...

CAT ASSAY 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml tube ...

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1. Remove gel slice contain DNA fragment and place in 10 volumes of: 300 mM NaOAc, pH 7.0 300 ml 1 M NaOAC, pH 7.0 1 mM EDTA 2 ml 500 mM EDTA, pH 8.0 698 ml ddH2O 2. Incubate at 22℃ for 30 min. Transfer gel slice to a fresh tube. 3. Place tube in a Dry Ice/Ethanol bath for 5 min. 4. Puncture the bottom of a 0.5 mL microcentrifuge tube with a needle. Place the gel slice into this tube. Place this tube inside a 1.5 mL microcentrifuge tube. 5. Centrifuge for 15 min. 6. Collect the Eluent from the 1