DNA提取与纯化

1. Add an equal volume (equal to sample volume) of P/C to sample. 2. Mix (shake don't vortex). 3. Take aqueous (upper) layer. (If dirty sample repeat Ph/Chl step until interface is fairly clean). 4. A ...

Phenol (removes protein) 1、add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol)2、vortex3、spin 2 minutes at 12000 rpm 4°C4、transfer supernatant to a fresh tube (avoi ...

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琼脂糖凝胶层析的技术数据 琼脂糖凝胶作为核酸检测主要也是理想材料，使用十分广泛，其理化性质一些参数如下表： （琼脂糖是琼脂内非离子型的组分，它在 0 ~ 4 ℃， pH 4~9 范围内是稳定的。） 名称、型号 ...

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This is a scaled up version of the Baron protocol which has been modified to achieve purity comparable to CsCl preps. I have not sorted out strain variations yet but HB101 works well. 1、Solutions Sol ...

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1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ . 2.pour into tube spin for 30 sec . 3.decant supernatant and resuspend pellet in 100 ml lysis solution . 4.add 200 ml alkaline SDS vortex well ...

After an aliquot of the PCR mixture is analyzed on an agarose gel the remainder of the reaction is concentrated by ethanol precipitation resuspended in buffer and subjected to a simultaneous fi ...

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近10年来，现代分子生物学技术越来越广泛地被用于人类疾病研究的诸领域，为了解病理状态下基因组DNA 的变化积累了新资料。目前认为，人类基因组并非人们想像的那样稳定，诸如基因重排、扩增、缺失，突变和DNA 甲基化类型改变等时有发生，这些改变对于基因表达和调控，以及疾病过程的发展与转归等方面均具有重要意义。 医院病理科档案中积存的大量石蜡包埋组织，是一个可靠的分子生物学研究的材料来源。 ...

动物样品的采集及处理方法 遗传学数据在野生动物和圈养动物的保护和管理中变得日益重要。在本文中，我们总结了一些能简便而有效地获得样品及数据的方法。 我们在采集样品之前，最好对将要进行的遗传学分析有所了解。然而，采集者并非都是研究者，当采集地与实验室有相当距离时，采集者常常要保存好样品直到有合适的接受者或存放地，这就要求采集者应具备一定的经验。 首先，所有样 ...

A. Culture Growth: 1.For BAC isolation for shotgun library construction: Pick a smear of colonies and inoculate 3 ml TB medium containing the appropriate antibiotic. Grow the culture for 8 hours 37 ...

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