DNA提取与纯化

Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing. Reagents •70% ethanol •3 M so ...

Submission Details: *Dr. Simon DawsoundefinedDepartment of BiochemistrundefinedUniversity of NottinghaundefinedU.K.*17:0undefined22/1/96. Database: Method/Protocol-->Spin column purification can be used to change buffers with ...

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Rapid elution of DNA from agarose gels James Movius Hahn Lab Last modified Sun Nov 1 1998 A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restr ...

Description This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley 1985). It is a useful way to avoid coprecipitation of proteins and accumulation of salt ...

In the hood: 96 well dish with bacteria titertech microtubes glass pipette Remove colonies from each well using the titertech and place them into the cover Pipette up and down to thoroughly mix the co ...

This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples. Materials •3M Sodium Acetate buffer pH 5.2 (store at 4 ℃) •Col ...

溶液配制 （一）LB培养基 每1000 ml加分析纯NaCl 10 g，蛋白胨10 g，酵母粉5 g，用ddH2O配制，再用10 M NaOH调pH至7.4（100 ml一般加450 μl），高温高压蒸汽灭菌15 min冷却后使用。 固体培养基加入琼脂1.5%。 10 M（或N）NaOH配制方法是称200 g NaOH加300 ml，搅拌充分溶解后定容至500 ml。 （二）溶液Ⅰ、 ...

Materials: phenol:chloroform (1:1) chloroform 1.Add an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. 2.Mix well. Most DNA solutions can be vortexed for 10 sec except f ...

甲基化研究对于基因的调控和肿瘤的发生有非常密切的关系。在一般正常的细胞中，基因调控区CpG岛处于非甲基化的状态。而当细胞发生癌变后，这些CG区域往往呈现甲基化状态。英国医学刊物《the Lancet》报道奥地利因斯布鲁克医学院的专家们早就可以通过检测大便中DNA的甲基化变化，把健康人的大便与结肠癌患者的大便区分开。大便标本中SFRP2 甲基化的程度是诊断结肠癌最灵敏的标记。研究人员发现 ...

提取DNA需要的仪器和试剂： ⑴ 1.5ml 离心管 ⑵ 移液管: 1000μl 200μl 40μl 各一支和移液管尖头: 100-1000μl 1-200μl 各一盒 ⑶ 微型滤柱（备用） ⑷ 小型旋转混合器一台 ⑸ 小型离心机: 可放入1.5ml 离心管和2ml收集管. ⑹ 恒温水浴 ⑺ 电冰箱: -20℃ 4 ℃ ⑻ 无水乙醇(自备) ⑼ 70 ...

Preparation of C. elegans Genomic DNA Protocol by Andy Fires Lab Modified my Min-Ho Lee and Sudhir Nayak This protocol is based on the original Fire lab protocol for isolating genomic DNA from C. eleg ...

Principle The nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped channel and trapped within a high salt cushion resting at the bottom of th ...

Part A: Purifying nuclear pellets. 1) Add 50-60ul fresh packed red blood cells (RBC) to 700ul PBS. Mix by inversion. 2) Add 700-800ul Lysis buffer. Close tube. Vortex briefly (2-5s) 3) Centrifuge for ...

Important Notes Before Starting • New users are strongly recommended to read the QIAGEN handbook before starting the procedure. •Before using the kit for the first time dissolve the lyophi ...

Isolation of Mouse genomic DNA Kill mouse by cervical luxation and dissect liver. Add to a 50ml tube and place on liquid nitrogen. Grind to a powder under liquid nitrogen using a mortar and pe ...

For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. Solutions Crush and Soak Solution 500 mM NH4 OAc ...

1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1-2h @ 37℃ with occassional swirling. 3. Phenol extract GENTL ...

Steve Hahn ...