DNA提取与纯化

Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml ...

1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin or 1mg/5ml. Optional: Use a 15ml conical tube with a loosened cap and a piece of tape to hold it in place. Shake at 2 ...

Author: Long-Cheng Li Source: Protocol Online Abstract: Simplified method for preparing G A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing re ...

Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this ...

Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice for isolating double stranded plasmid-based templates for the Sequenase Dye-Labeled Terminator Sequencing Reactions. A. ...

1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc pH 7.0 300 ml 1 M NaOAC pH 7.01 mM EDTA 2 ml 500 mM EDTA pH 8.0698 ml ddH2O2. Incubate at 22 ℃ for 30 min. Transfer gel ...

Important : Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day.1. Add 600 m l of 50 mM ...

1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks shake & pour into tubes.3. Spin down the cells @ 1K RPM for 5'.4. Resuspend in 1 ml TBS-.5. Transfer to 1.5 ml tubes.6. Spin down @ 14K RPM ...

Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly homogenates with 100 ng/ml ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also DNA RNA double isolation procedure if both DNA and RNA are needed)Reage ...

Materials:phenol:chloroform (1:1) chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high ...

This is a modification of a salting out procedure as described by Miller et al. (1988) evaluated at the DNA Laboratory Medical School Malta. When analysed by spectrophotometry 95% of extracted genomic ...

SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g Ureaup to 500 ml Q 250 ml 10X TBE 120.8 g Acrylamideup to 500 ml Q 4.1 g BISup to 500 ml Q2.5 M NH4 OAc19.2 g NH4 OAcup t ...

Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk• NaI solution• New WashProcedure:1) Run digested DNA out on agarose gel slowly (70 V on BioRad gel)2) Use lon ...

Materials: RPMI 1640 medium fetal calf serum (FCS) 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents Best.-Nr. 295892) cell cuture flask Phythemaglutinin PHA-L (Seromed M 5030) CO2 cell cul ...

1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampicillin or appropriate antibiotic. Shake at 250 RPM overnight.2. Centrifuge cells in a Sovall GSA (250 ml)or SLA-3000 (50 ...

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Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume to remove protein contaminants or to purify plasmid DNA su ...

Glass wool method: Run TAE agarose gel and cut the appropriate band out with a clean razor blade. Poke a small hole with hot needle on the bottom of an eppendorf tube and jam the hole with a little of ...

TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose gel piece containing the desired DNA fragmentSupplies: 1.Micropipetter and tips 2.Microcentrifuge and tubes 3.Spatu ...