DNA基础知识

Developed for microarray-based comparative genomic hybridization.Genomic DNA can be labeled with a simple random-priming protocol based on Gibco/BRL's Bioprime DNA Labeling kit though nick translation ...

PrincipleSingle-strand conformation polymorphism (SSCP) technique is a simple and efficient means to detect any small alteration in PCR-amplified products. It is based on the assumption that subtle nu ...

Genomic DNA will be randomly primed with a sequence tagged oligonucleotide for 2 cycles. This will create random genomic products with a specific tag at both ends. These products will then be amplifi ...

Genomic DNA will be randomly primed with a sequence tagged oligonucleotide for 2 cycles. This will create random genomic products with a specific tag at both ends. These products will then be amplifi ...

The major considerations in using alcohol to precipitate DNA are:Temperature: -20°C is optimal but 0°C can be used for 20 ng/mL2) Amount: For small amount of DNA ( i.e. too little to reliably see a pe ...

Reagents:Lysis Buffer: (10 mM Tris pH8 100 mM NaCl 25 mM EDTA 0.5%SDS) store at room temp.10 mg/mL Proteinase K: 100 mg (Roche) + 10 mL buffer (10 mM Tris pH8.0 20 mM CaCl2 50% (v/v) glycerol) store ...

SolutionsPBSCell lysis buffer: 10 mM Tris pH8 100 mM NaCl 25 mM EDTA 0.5% (w/v) SDS 0.5 mg/mL proteinase KFixative: 1 volume glacial acetic acid + 3 volumes methanolTrizol reagent75% EtOH made with ...

The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however its high rates of DNA ...

Epigenetics is the study of heritable changes in gene expression. Chromatin immunoprecipitation (ChIP) and methylation status analysis of genes have been applied to the study of epigenetic modificatio ...

Make sure that there is enough dCTP and TTP (or dGTP and dATP) in the hot room Set up your PCR master mix. Remember that you need to do a reaction for dCTP and TTP for each sample 10xPCR Buffer 2.5u ...

Ancient DNA extraction from bones and teeth.pdf ...

Non_destructive_Biotechniques_2004art.pdf

Tissue preparation We used FFPE tissue blocks of Non-small cell lung cancer obtained from Pathology department of Kerman Medical University which were fixed from 2004 to 2006. Before outset of our exa ...

Non_destructive_Biotechniques_2004art.pdf

Meyerowitz et. al. (~1987ish) A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysacchar ...

Nuclei isolation modified from Hamilton Kunsch and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless ...

Meyerowitz et. al. (~1987ish) A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysacchar ...

Nuclei isolation modified from Hamilton Kunsch and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless ...

Use fresh or Lyophilize the leaf tissue. Grind about 5 g leaf tissue with mortar and pestle in liquid N2 transfer the powder into an 50 ml polypropylene tube (50 ml) and store at -20°C. Add 25 ml of ...

This protocol originated in Murray and Thompson (1980) and then was modified by Richard Jorgensen and finally published in Wagner et. al 1987.Start with about 10 grams fresh needles. Chop into short ...