DNA基础知识

Restriction Map and Multiple Cloning Site of pEGFP-N2. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Nhe I site cannot be used for fusions since it c ...

Map of pLP-EGFP-C1 Vector. Unique restriction sites are shown in bold. ...

Restriction Map and Multiple Cloning Site of pbgal-Control. Unique restriction sites are in bold. ...

Restriction Map and Multiple Cloning Site of pbgal-Basic. Unique restriction sites are in bold. ...

Isolation of DNA from Acrylamide Gels1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is correct for most applications. 2. Run out DNA fragments. For fragments greater than ...

Oligonucleotide PurificationSteve HahnLast modified 10/16/98Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed m ...

DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver NitrateSource: Contributed by Mohammad Reza Abbaszadegan et al.Abstract: Single strand conformational polymorphism (SSC ...

Electro-elution of nucleic acids from agarose and polyacrylamide gel slides.PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped ...

Electro-elution of nucleic acids from agarose and polyacrylamide gel slides.PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped ...

Recovering DNA from agarose gels Paul N. Hengen Ph.D. (July 14 1999) Introduction========Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.mol ...

Materials: TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)Frozen agarose gel piece containing the desired DNA fragmentSupplies: Micropipetter and tips Microcentrifuge and tubes Sp ...

Rapid elution of DNA from agarose gelsJames Movius Hahn Lab Last modified Sun Nov 1 1998A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restrict ...

Materials: TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)Frozen agarose gel piece containing the desired DNA fragmentSupplies: Micropipetter and tips Microcentrifuge and tubes Sp ...

PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKSCut 10-20X 10 um sections of formalin fixed paraffin samples into eppendorf tubes. Add 1 ml xylene mix incubate at 55 C for 15 mins. Release pressure spin d ...

1. Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55EC for 5 min or until the gel dissolves.3. ...

1. Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55EC for 5 min or until the gel dissolves.3. ...

DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is prefe ...

DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is prefe ...

DNA Extraction from Archival Formalin-fixed Paraffin-embedded Tissue SectionsAuthor: Shi et al.Source: Contributed by APostodocAbstract: Describes two methods of extracting DNA from archived paraffin- ...

DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONSThis is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologist ...