数据库

Optimal protein analysis requires unfixed tissue samples. We suggest handling the brain tumor tissue sterilely and coldly (on ice) for as short time as possible prior to processing, but for no more than 8 h. This simple protocol results in apparently intact morphology, immunoreactivity, pro ...

This chapter briefly reviews the role of twin studies and study designs based on using twins in different settings. In the Nordic countries, twin registers and cohorts have existed already for many decades. These are a unique resource for scientific studies; a major strength being their unsele ...

The storage of the different microorganisms over long periods is necessary to ensure reproducible results and continuity in research and in biomedical processes and also for commercial purposes. Effective storage means that a microorganism is maintained in a viable state free of cont ...

Maximal extraction and solubilization of protein from diseased or healthy tissue is important to make the whole protein complement available for proteomic analysis. It also helps to maximize reproducibility and to minimize waste. Minimal degradation of the protein amino acid backb ...

Access to high-quality fresh frozen tissue is critical for translational cancer research and molecular �diagnostics. Here we describe a workflow for the collection of frozen solid tissue samples derived from fresh human patient specimens after surgery. The routines have been in oper ...

A cervical cytology biobank (CCB) is an extension of current cytopathology laboratory practice consisting in the systematic storage of Pap smears or liquid-based cytology samples from women participating in cervical cancer screening with the explicit purpose of facilitating fu ...

Two vital pre-requisites for genetic epidemiology have been fullfiled during the past decade and have led to a virtual explosion of knowledge concerning disease risks. Reliable databases over genetic variation derived from, e.g. the HUGO and HapMap projects, coupled with technologic ...

A biobank may be defined as the long-term storage of biological samples for research or clinical purposes. In addition to storage facilities, a biobank may comprise a complete organization with biological samples, data, personnel, policies, and procedures for handling specimens and pe ...

When establishing a biobank, there are a number of issues to consider with significant impact on the outcome of your efforts and the quality of your work. Some of the most relevant are: (1) study design, (2) the size and composition of your cohort, (3) the nature and desired quality of the biological material, ...

The first part of the chapter describes the Icelandic Genealogical Database, how it was created, what it contains, and how it operates. In the second part, an overview of research accomplished with material from the database is given.

The Swedish Multi-generation Register consists of data of more than nine million individuals, with information available on mothers in 97% and on fathers in 95% of index persons. Index persons are confined to those born from 1932 onwards and those alive on January 1, 1961. This register is a unique res ...

The PRINT� (pattern replication in non-wetting templates) process has been developed as a simple, gentle way to pattern films or generate discrete particles in arrays out of either pure biological materials or biomolecules encapsulated within polymeric materials. Patterned films ...

Microcontact printing (μCP) is a useful technique for transferring certain molecules onto surfaces with high spatial resolution using elastomeric stamps. The stamp for μCP is fabricated by replica molding from a master made by microlithography. After wetting with a type of material as an ...

This chapter describes a protocol for forming open endothelial tubes in vitro and quantifying their permeability to macromolecules. These tubes consist of confluent monolayers of human microvascular endothelial cells in perfused microfluidic collagen gels. The cylindrical ...

This book chapter discusses recent advances in the fabrication of microscale surface patterns using chemical vapor deposition polymerization. Reactive poly(p-xylylene) (PPX) coatings are useful for their ability to immobilize specific biomolecules, as determined by the PPX f ...

Bioinformatics has become an increasingly important tool for molecular biologists, especially for the analysis of microarray data. Microarrays can produce vast amounts of information requiring a series of consecutive analyses to render the data interpretable. The direct outp ...

Photolithographic patterning of proteins on surfaces has been used extensively in the past to define cell adhesion domains with micrometer-scale resolution. However, photolithographic patterning is not amenable to depositing several different proteins on the same surface. We ...

In this chapter, we describe the fabrication and use of microfluidic capillary systems (CSs) made in soft, transparent polydimethylsiloxane (PDMS). Sixteen microfluidic CSs, each containing a loading pad, a microchannel, and a capillary pump are engraved in a single PDMS chip. The CSs are us ...

We have developed a combinatorial platform for fabricating tissue scaffold arrays that can be used for screening cell–material interactions. Traditional research involves preparing samples one at a time for characterization and testing. Combinatorial and high-throughput ( ...

There is a growing interest on the fabrication of bacteria and virus microarray owing to their great potential in many biological applications ranging from diagnostic devices to advanced platforms for fundamental studies on molecular biology. Over the past decade, a number of studies wi ...