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        丁香实验推荐阅读
        Modification of Carbon Nanotubes for Gene Delivery Vectors

        The surface modification of carbon nanotubes (CNTs) can be tailored to allow the formation of a complex between these potential carriers with DNA. In this chapter, protocols developed in our lab to prepare transfection vectors through the modification of MWCNTs are described. The protocol ...

        丁香实验推荐阅读
        Noncovalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs

        The successful delivery of hydrophobic drugs to cellular targets continues to present challenges to the pharmaceutical industry. The advances made by nanotechnology have generated new avenues for selectively loading, delivering, and targeting these drugs to their biological ...

        丁香实验推荐阅读
        Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy

        Silver and large gold nanoparticles are more efficient scatterers than smaller particles, which can be advantageous for a variety of single-particle-based sensing and spectroscopic applications. The increased susceptibility to surface oxidation and the larger surface area of ...

        丁香实验推荐阅读
        Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging

        Iron oxide nanoparticles, due to their exceptional magnetic property, biocompatibility, and biodegradability, have long been studied as contrast agents for magnetic resonance imaging (Xie et al., Curr Med Chem 16(10):1278–1294, 2009; Xie et al., Adv Drug deliv Rev 62(11):1064–1079, 2010). ...

        丁香实验推荐阅读
        Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles

        We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inor ...

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        Zwitterion Siloxane to Passivate Silica Against Nonspecific Protein Adsorption

        Passivating surfaces against protein adsorption is important for many biotechnological applications. Current approaches have been exploiting the use of zwitterions instead of poly(ethylene glycol) (PEG). Commonly used zwitterions are polymeric and are grafted onto surfa ...

        丁香实验推荐阅读
        Stability and Aggregation Assays of Nanoparticles in Biological Media

        Colloidal stability of nanoparticles in biological media is crucial to preserve their utility as aggregation often leads to undesirable biological response. A quantitative measurement of nanoparticles aggregation in solution would provide a valuable assessment of colloi ...

        丁香实验推荐阅读
        Immunocytochemistry, Electron Tomography, and Energy Dispersive X-ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Res

        The cryosectioning technique is an alternative method for preparing biological material for Transmission Electron Microscopy (TEM). We have applied this technique to study the mechanism of cell internalization of stimuli-responsive polymeric nanogels exploited as cargo n ...

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        Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment

        Nanoparticles (NPs) are being implemented in a wide range of applications, and it is critical to proactively investigate their toxicity. Due to the extensive range of NPs being produced, in vitro studies are a valuable approach for toxicity screening. Key information required to support in v ...

        丁香实验推荐阅读
        Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Amon

        The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis ...

        丁香实验推荐阅读
        Formation and Characterization of the NanoparticleProtein Corona

        Over the last decade the existence of “the corona,” a natural interface between nanomaterials and living matter in biological milieu, evolved from a vague concept into broadly recognized fact. This robust shell arises (to some extent) on the surface of all nanoparticles (NPs), even the ones des ...

        丁香实验推荐阅读
        Electrochemical Measurements of DNA Melting on Surfaces

        Thermal denaturation, or melting, measurements are a classic technique for analysis of thermodynamics of nucleic base driven associations in solution, as well as of interactions between nucleic acids and small molecule ligands such as drugs or carcinogens. Performed on surface-im ...

        丁香实验推荐阅读
        Functionalization Protocols of Silicon Micro/Nano-mechanical Biosensors

        Functionalization is a key element in biodetection technologies such as micro/nano-mechanical sensors. Since assay sensitivity and stability drastically depends on a proper bioreceptor immobilization, the sensing surface must be first chemically modified with uniform, w ...

        丁香实验推荐阅读
        Surface Modifications by Polymers for Biomolecule Conjugation

        Polymeric coatings, usually referred as tridimensional chemistries, provide homogenous surface derivatization methods presenting a high reactive group concentration and resulting in an increased binding capacity of targets. Furthermore, they act as linkers distribut ...

        丁香实验推荐阅读
        Reliable Methods for Silica Coating of Au Nanoparticles

        The inherent properties of silica, such as optical transparency, high biocompatibility, chemical and colloidal stability, controllable porosity, and easy surface modification, provide silica materials with a tremendous potential in biomedicine. Therefore, the coating of Au ...

        丁香实验推荐阅读
        Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates

        Quantum dots (QDs) are well-established as photoluminescent nanoparticle probes for in vitro or in vivo imaging, sensing, and even drug delivery. A critical component of this research is the need to reliably conjugate peptides, proteins, oligonucleotides, and other biomolecules to QDs ...

        丁香实验推荐阅读
        Water-Solubilization and Functionalization of Semiconductor Quantum Dots

        Semiconductor quantum dots (QDs) are highly fluorescent nanocrystals that have abundant potential for uses in biological imaging and sensing. However, the best materials are synthesized in hydrophobic surfactants that prevent direct aqueous solubilization. While several m ...

        丁香实验推荐阅读
        Conjugation of Nanoparticles to Proteins

        Nanoparticle–protein conjugates hold great promise in biomedical applications. Diverse strategies have been developed to link nanoparticles to proteins. This chapter describes a method to assemble and purify nanoparticle–protein conjugates. First, stable and biocompa ...

        丁香实验推荐阅读
        DNA Conjugation to Nanoparticles

        Nanoparticle–DNA (NP–DNA) conjugates have been highlighted due to their versatility in diverse science and engineering fields. The protocol of DNA conjugation to gold nanoparticles (AuNPs), which are among the most popular NPs in bio-applications, is thus described here. This proto ...

        丁香实验推荐阅读
        Preparation of 2 nm Gold Nanoparticles for In Vitro and In Vivo Applications

        Gold nanoparticles have been a versatile tool in recent years for the exploration of biological systems. However, challenges with purification and adequate surface coverage limit the biocompatibility of gold nanoparticles. Here, we describe a detailed procedure for the synthesi ...

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