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Abstract Table of Contents Materials Literature Cited Abstract In this unit, we describe a protocol for regulated gene expression in primary endodermal organoid culture using retroviral vectors. The study of gene function in endodermal epi

Abstract Table of Contents Materials Figures Literature Cited Abstract The importance of laboratory mice in experimental microbiology and biomedical research is indisputable, and their care and handling i

Abstract Table of Contents Materials Figures Literature Cited Abstract West Nile virus (WNV) is a member of the Flaviviridae family of enveloped, single?stranded, positive?sense RNA viruses. WNV, an emergin

Abstract Table of Contents Materials Literature Cited Abstract Listeria monocytogenes causes foodborne disease in humans that ranges in severity from mild, self?limiting gastroenteritis to life?threatening systemic infections of the blood,

Abstract Table of Contents Materials Figures Literature Cited Abstract Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties, e.g., incr

Abstract Table of Contents Materials Figures Literature Cited Abstract The Western world is in the midst of an epidemic of obesity, which is the cause of severe clinical complications such as type 2 diabe

Abstract Table of Contents Materials Figures Literature Cited Abstract Mouse models recapitulate many symptoms of Rett Syndrome, an X?linked disorder caused by mutations in methyl?CpG?binding protein 2 (M

Abstract Table of Contents Materials Figures Literature Cited Abstract Controlled proliferation of cardiomyocytes remains a major limitation in cell biology and one of the main underlying hurdles for true

Abstract Table of Contents Figures Literature Cited Abstract Living organisms grow, differentiate, reproduce, and respond to their environment via specific and integrated interactions between biomolecules. The investigation of molecular in

Abstract Table of Contents Materials Figures Literature Cited Abstract The proteasome plays a central role in eukaryotic cells since it is responsible for the degradation of specific proteins involved in

Abstract Table of Contents Materials Figures Literature Cited Abstract Isothermal titration calorimetry (ITC) is perhaps the most rigorous commercially available method for characterizing protein?ligand i

Abstract Table of Contents Figures Literature Cited Abstract The specific interaction of biological molecules with one another is fundamental to the biochemistry of all living things. Equilibrium sedimentation is a classic method of bioche

Abstract Table of Contents Figures Literature Cited Abstract The biological function of many proteins involves reversible interactions with other proteins, nucleic acids, or other non?protein ligands. Such interactions play many different

Abstract Table of Contents Literature Cited Abstract This overview defines some of the terminology used in the study of protein?protein interactions and also describes the basis of equilibrium kinetic assays (i.e., defining necessary assumptions, terms and equations).

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes group?specific modifications of amino groups. These reactions remain valid tools for early?stage evaluation of st

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a number of methods for modifying cysteine residues of proteins and peptides by reduction and alkylation procedur

Abstract Table of Contents Materials Figures Literature Cited Abstract One of the most versatile and accessible types of isolated tissue used in pharmacology is the isolated blood vessel. Arteries and vei

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes assays for measuring the binding of radioligands to two major types of receptors for 5?hydroxytryptamine (5?HT or

Abstract Table of Contents Materials Figures Literature Cited Abstract The N?methyl?D?aspartate subtype of glutamate receptor is unusual in that it requires two endogenous agonists for activation. Thus, in

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit provides an introduction to the binding of specific ligands to K+ channels that are expressed in a number of different prepar