实验基础

Protocol for quick tests of restriction enzyme activity in novel species Belavin P. A. Dedkov V.S. Degtyarev S.Kh. A method to detect restriction endonucleases in bacterial colonies. (1988) Applied Bi ...

Getting The Most Out Of Your BugsNative lysis is a staple protocol in practically every biochemistry lab yet there is significant variability in the procols I have read. The intention is to liberate t ...

OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step. Updated Protocol: Blackburn Lab: Quick and Easy Yeast Colony PCR v2.0 MaterialsStandard PCR machine tub ...

Intergeneric Conjugation and OverlayDescription Transfer of plasmid/cosmid DNA from a host strain e.g. E.coli ET12567 to the recipient strain e.g. Streptomyces coelicolor. Approx. Duration: Conjugati ...

Small Scale Plasmid Isolation (Mini / Maxi Prep)Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria normally E.coli. An overnight culture of cells is lysed and t ...

Description Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template double stranded DNA (dsDNA) to single stranded DNA (ssD ...

Description Transform E.coli cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into E.coli). Approx. Duration: Preparation ~30 minutes Making the competent cells ~ ...

SummaryCleaner stocks of T7 that concentrates and purifies T7 bacteriophage. ProtocolGrow 100mL of permissive cells to a density of 108 to 109 cells/ml at 30°C in a rotary shaking water bath. Inoculat ...

Materials50mL 7H9 mycobacterial medium + 3mL per transformation 102mL 10% glycerol (possibly a few mL more if you are doing many transformations) 400mL PBS + 0.1% Tween 80 per transformation electropo ...

Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 20% sterile glycerol at -20°C. It will last for man ...

Summary How to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep. ProtocolAdd 5mL fresh overnight (BL21 if wild-type ...

大多数豆科植物与真菌共生。丛枝菌根真菌（AM）对磷（P）的吸收和根瘤菌对氮（N2）的固定具有重要的农学和生态学意义。植物-AM真菌-根瘤菌三个共生如何高效吸收营养的机制受到很多关注。AM真菌和根瘤菌能够有效地增加固氮和植物对土壤中磷的吸收，但这破坏了根部阴阳离子平衡，过多的阳离子需要从根部分泌出来才能达到平衡，H+是分泌的最主要阳离子。然而，无论根瘤能够直接释放H+引起土壤的酸化， ...

Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlycerol 20mlAdjust PH to 7.0 after totally solved.Ster ...

Lenti-Virus Protocol张端午A. For packing virus in 6-well plate: (if in 12-well plate reduce all to 1/2)1. Mix the following plasmids in a 1.5 ml eppendorf tube1.5 μg Lenti-vector + 1.5 μg packing plasmid ...

1腺病毒的包装，纯化和感染技术背景：Adeasy 系统利用了腺病毒具有的高感染能力，可高度浓缩等优点，并破坏了腺病毒基因组中的早期基因E1，使之成为较为安全、高效的病毒载体。利用带有E1 基因的293 细胞作为包装细胞，通过倍比扩增，富集病毒颗粒，并通过CsCl 梯度离心，透析进行分离纯化。对绝大多数的细胞株可以达到近乎100％的感染效率。但需要指出的是Adeasy 同样具有所有病毒载体或转染试剂 ...

甲型流感病毒抗原(Flu A-Ag)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于检测血清，血浆及相关液体样本中甲型流感病毒抗原(Flu A-Ag)水平。实验原理： 本试剂盒采用双抗体夹心酶联免疫法（ELISA）测定标本中甲型流感病毒抗原(Flu A-Ag)。用纯化的甲型流感病毒抗体包被微孔板，制成固相抗体，可与样品中甲型流感病毒抗原(Flu A-A ...

犬传染性肝炎病毒抗原(ICHV-Ag)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定犬血清，血浆及相关液体样本中传染性肝炎病毒抗原(ICHV-Ag)的含量。实验原理： 本试剂盒采用双抗体夹心酶联免疫法（ELISA）测定标本中犬传染性肝炎病毒抗原(ICHV-Ag)。用纯化的犬传染性肝炎病毒(ICHV)抗体包被微孔板，制成固相抗体，可与样品中传染性肝 ...

犬冠状病毒酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定犬血清，血浆及相关液体样本中冠状病毒水平。实验原理： 本试剂盒采用双抗体夹心酶联免疫法（ELISA）测定标本中犬冠状病毒。用纯化的犬冠状病毒抗体包被微孔板，制成固相抗体，可与样品中冠状病毒相结合，经洗涤除去未结合的抗体和其他成分后再与HRP标记的冠状病毒抗体结合，形成抗体-抗原-酶标抗体复合 ...

犬瘟热病毒抗体(CDV-Ab)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定犬血清，血浆及相关液体样本中瘟热病毒抗体(CDV-Ab)的含量。实验原理： 本试剂盒采用双抗原夹心酶联免疫法（ELISA）测定标本中犬瘟热病毒抗体(CDV-Ab)。用纯化的犬瘟热病毒抗原包被微孔板，制成固相抗原，可与样品中犬瘟热病毒抗体(CDV-Ab)相结合，经洗涤除去 ...

犬瘟热病毒抗原(CDV-Ag)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定犬血清，血浆及相关液体样本中瘟热病毒抗原(CDV-Ag)的含量。实验原理： 本试剂盒采用双抗体夹心酶联免疫法（ELISA）测定标本中犬瘟热病毒抗原(CDV-Ag)。用纯化的犬瘟热病毒(CDV)抗体包被微孔板，制成固相抗体，可与样品中犬瘟热病毒抗原(CDV-Ag)相结合， ...