Immobilization of ligands on cuvette surfaces and measure the interactions of ligand which is immobilized on the cuvette and the ligate which is added to the immobilized cuvette. Immobilization of lig ...
在酶标记的溶液中,出现的是各种交联物的混合物,有酶-抗体、酶-抗体-酶、抗体-抗体、酶-酶,甚至还有游离的酶和抗体。 在这中间,除了抗体-酶和酶-抗体-酶外,其它都应该给予除去。 1.50%饱和硫酸铵沉淀法。 此法只能除去游离的酶及酶-酶聚合体。而不能除去其它不需要者。但是此法对酶标抗体的损耗少。&nb ...
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ...
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一、概况 噬菌体展示技术(Phage Display Technology)在80年代中期产生, Smith G第一次描述了在丝状噬菌体表面展示外源多肽片段的原理。该办法主要是用DNA重组技术将大量的多肽编码顺序导人噬菌体的衣壳蛋白基因中,从而使表达出的各种多防以与PIII或PVIII蛋白融合的形式出现在噬菌体的表面,即首先构建噬菌体多肽展示文库。10多年来在构建噬菌体展示多肽文库方面以及用生物 ...