抗体抗原实验

This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70 monocytes. This cell suspension is than use ...

OUTLINEThis modification of peritoneal macrophage preparation is designed for a subsequent thrombophagocytosis test. PROTOCOLSacrifice a mouse (use dry ice CO2) Sink a mouse in the 5 water solution o ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Source of unwanted staining besides poor knowledge of the antibody reactivity and malice is due to: Endogenous enzymes or fluorochromes. Endogenous biotin. Endogenous antibody binding activity (Fc rec ...

Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi et al. 1988 J. Histochem. Cytochem. 36:511-514). This m ...

VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP) (http://ntp.niehs.nih.gov/) interdisciplinary research programs and DIR research using new technologies in ...

Rationale: The use of DNAse to improve nuclear antigen staining has been published long before the AR era 1 2.DNAse treatment is currently suggested as an unmasking technique for incorporated BrdU nuc ...

Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections refer to the respective ''indirect'' techniques. T ...

Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections refer to the respective ''indirect'' techniques. T ...

You will need the following reagents:- Antibodies Normal Swine Serum (NSS) 1:5 in Tris buffer. 1°: antibody diluted in 1/20 NSS/Tris. 2° antibody diluted in 1/20 NSS/Tris. Streptavidin/biotin complex ...

1. Dissect brains in Drosophila Ringers solution. 2. Fix 20'' (1hr max) in a 0.5ml microfuge tube with 5 formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipe ...

1. Dissect brains in Drosophila Ringers solution. 2. Fix 20'' (1hr max) in a 0.5ml microfuge tube with 5 formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipe ...

OverviewR&D Systems provides monoclonal polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed and optimized by R&D Systems'' Immunohistochemical ...

MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Seconda ...

Procedure:1) Fix tissues for 3 hr on ice in 4 formaldehyde (2.5 ml of Polysciences #18814 made up to 10 ml in 80 mM NaPO4 pH 6.8 containing 0.2 gm of sucrose). Wash for 2 hr in several changes of c ...

Objective:Bgal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity. Procedure ...

Objective:Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary a ...

Dechorionate directly on apple juice/agar plates with 50-60 chlorox; embryos will float to the surface after 2-3''. Collect embryos on a Millipore device with a nitex filter; rinse well with 0.1 Trito ...

Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS replace with methanol store at -20oC. Rehydrate by slowly adding TBS over a ten minute period. Once sample ...