抗体抗原实验

General remarks:Ultrathin methacrylate-(e.g.Lowicryl HM20 K4M Monostep polar and nonpolar or LR-White) or epoxy (Epon/Araldite Spurr) sections are transferred with a loop on poly-L-lysine coated round ...

DescriptionThis procedure describes how to process cells for immunofluoresence microscopy. Procedure1. Sterilize coverslips under UV light for 20-30ma. To increase cell adherence treat coverslips with ...

Antibodies to small peptides have become an essential tool in life science research with applications including gene product detection and identification protein processing studies diagnostic tests pr ...

In order to decrease the amount of nonspecific staining it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeas ...

The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO Richmond California) (see Table 1). On West ...

This is the protocol to use with the Botstein anti-actin rabbit antibodies.Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37 formaldehyde (best grade) and incubate on the ...

Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37 formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. Spin down cells 2Kx3min. and resuspend in ...

This protocol describes our method for preparing cells for immunofluorescence in which all incubations and washes are performed in microtiter dishes. Two dishes can be processed simultaneously. The la ...

DescriptionThis is the method for direct immunofluorescence labeling; that is the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. Procedure ...

Direct Immunofluorescence Staining cells with antibodies directly linked to fluorochromes is known as direct immunofluorescence (DIF). DIF lends itself to multicolour experiments where a cell suspensi ...

This protocol is recommended for rat tissue fixed by paraformaldehyde-picric acid perfusion Tissue preparation:Subjects transcardially perfused with heparanized saline (0.9 w/v NaCl) followed by 4 w/v ...

BackgroundThis is the method for indirect immunofluorescence labeling; that is the antibodies do not have the fluorescent dye attached. Indirect labeling is more involved than direct labeling. If you ...

Supplies:Ethanol 100 USP (highest quality)FACS Staining Buffer (1XPBS w/ 3 calf serum 0.05 azide--filtered) ―Dilute staining antibodies in BufferDNAse (Sigma D-5025 Bovine Pancreas)RNase (Boehringer 2 ...

Chemicals and equipmentsActin Buffer ( 20 mM KPO4 10 mM PIPES 5 mM EGTA 2 mM MgCl2 pH 6.8) Assay buffer (Actin Buffer with 3.7 formaldehyde 0.1 triton 200X TRITC- Phaloidin from 50 mM stock.) DB Prep ...

The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample whether it is from the serum of an immunized animal or the cell supernatant from growing h ...

The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites capable of binding to antibody since at least ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

MATERIALS10ml sterile syringes (one for each mouse) 20 gauge needles (one for each mouse) Phosphate Buffered Saline pH 7.2: PBS 1X (GibcoBRL Cat. No. 20012-027) Isoflurane for anesthetizing 70 Ethano ...

This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70 monocytes. This cell suspension is than use ...