抗体抗原实验

IntroductionGreen fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien 1996; Tsien 1998). The validation and inte ...

IntroductionRNA-protein interactions play important roles within the cell. Using a variation of the widely-used chromatin immunoprecipitation (ChIP) assay the potential association of cellular RNAs an ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Conjugation of carrier (BSA) to peptide1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate pH 7.0.2. Dissolve MBS-SMCC @ 25mg/ml in NNdimethylformamide.3. Mix 20ml SMCC and 200ml BSA (500mg:4000mg or 1: ...

The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recomb ...

There are many different types of ELISAs which can detect the presence of protein in serum or supernatent. One of the most common types of ELISA is the so-called "sandwich ELISA." It is termed this be ...

You will need the following solutions: PBS (Phosphate-Buffered Saline Ca/Mg free) :- Solute1x10xNaCl8g80gKCl0.2g2gNa2PO4 (anhydrous)1.15g11.5gKH2PO4 (anhydrous)0.2g2gN.B.: Na2HPO4.2H2O can also be use ...

Sensitize a 96-well microtiter plate with purified antigen. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 1 ...

Immunoassays are a powerful technique for detecting and measuring antigens and antibodies. Immunoassays can be classified three ways based on the steps involved: antibody capture antigen capture two ...

OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) ( ...

This protocol can be used for detection of gangliosides.Spicific antibodies to gangliosides are added to cells and allowed to bind to the cell surface. The nonbound antibody is washed away and an enzy ...

Reagents1. DMEM:10% FBS2. DMEM:10% FBS:1% HEPES (DFH)3. 10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g Potassium dibasic phosphateDissolve in 900 ml di-H2O. Ch ...

PBSPBS:0.1% BSA:0.05% Tween 20 (PBT)PBS:2% BSA10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g Potassium dibasic phosphateDissolve in 900 ml di-H2O. Check pH and adjust to ...

ReagentsSodium Borohydride (NaBH4) - 1 mg/mlCarbonate:Bicarbonate Buffer pH 9.6 Sodium Periodate (NaIO4) - 1 mM 2.14 mg/10 ml Acetate Buffer 10mM Acetate Buffer pH 4.50.82 g sodium acetate in 90 ml di ...

Immunocytochemistry is the name given to methods that use antibodies to detect the location of proteins within cells. The antibodies bind specifically to the protein being investigated. With electron ...

This method has been used successfully in our laboratory since 1994. The protocol assumes all users will be familiar with routine TEM preparation methods. An important application of this method was m ...

Preparation of Colloidal Gold Conjugates.Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years colloidal gold particles have be ...

Immunocytochemistry at the ultrastructural level (TEM)Results obtained from immunocytochemical labeling can often differ from what was expected. Sometimes this may result in the formulation of new the ...

Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate empty into sink hit onto tow ...