抗体抗原实验

Biological specimens are embedded in resin so that they can be thin sectioned. Many different resin formulas are currently available and all have been used for post-embedding labeling (i.e. where anti ...

Equipment and chemicalsZeiss inverted microscope eppendolf microinjector eppendolf micromanipulator (in Dr. Murphy’s lab)Eppendolf femtotips 100mM cAMP in PMDB buffer PM Buffer.Preparing Cellsa. Wash ...

以酶联免疫反应与碱性去磷酸酶呈色反应侦测杂合讯息 进行杂合反应时所使用的探针若为放射性物质所标定者，杂合讯息可经由放射显影术 (autoradiography) 侦测出來；若为DIG所标定者，则需借助anti-DIG抗体与碱性去磷酸酶 (alkaline phosphatase AP) 的conjugate，并利用碱性去磷酸酶的酵素活性转现杂合反应讯息。 目前常用來进行碱性去磷酸酶呈色 ...

人细胞因子分装ELISA试剂盒货号品名方法规格价格备注F00010血管紧张素转化酶(Human ACE)ELISA48T/96T2000/3000进口分装F00020激活素A(Activin A)ELISA48T/96T2000/3000进口分装F00021雄激素结合蛋白(ABP)ELISA48T/96T1800/2800进口分装F00022促肾上腺皮质激素(ACTH)ELISA48T/96T ...

小鼠细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F10000小鼠血管紧张素转化酶(mouse ACE)ELISA48T/96T2000/3000进口分装F10001小鼠雄激素结合蛋白(mouse ABP)ELISA48T/96T2000/3000进口分装F10002小鼠促肾上腺皮质激素(mouse ACTH)ELISA48T/96T1800/2800进口分装F10010小鼠Acti ...

大鼠细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F15000大鼠Active Caspase-3ELISA48T/96T2000/3000进口分装F15010大鼠Active Caspase-7ELISA48T/96T2000/3000进口分装F15020大鼠Active Caspase-8ELISA48T/96T2000/3000进口分装F15030大鼠激活素A(rat Acti ...

兔细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F19011兔子脂联素(rabbit adiponectin)ELISA48T/96T1800/2800进口分装F19021兔骨成型蛋白-2(rabbit BMP-2)ELISA48T/96T2000/3000进口分装F19031兔子脑钠素/脑钠尿肽(rabbit BNP)ELISA48T/96T2000/3000进口分装F19051兔 ...

猪细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F25000猪促肾上腺皮质激素(Swine ACTH)ELISA48T/96T2000/3000进口分装F25001猪脑钠素/脑钠尿肽(Swine BNP)ELISA48T/96T2000/3000进口分装F25002猪III型胶原(Swine Collagen Type III)ELISA48T/96T1800/2800进口分装F25 ...

网址:www.westang.com 电话：021-65333639 55229872豚鼠细胞因子分装ELISA试剂盒F26100豚鼠内皮素-1(Guinea pig ET-1)ELISA48T/96T2000/3000进口分装F26110豚鼠表皮生长因子(Guinea pig EGF)ELISA48T/96T1800/2800进口分装F26120豚鼠促卵泡素(Guinea pig FSH)EL ...

网址:www.westang.com 电话：021-65333639 55229872狗细胞因子分装ELISA试剂盒F28100狗脂联素(Canine adiponectin)ELISA48T/96T2000/3000进口分装F28110狗C-反应蛋白(Canine CRP)ELISA48T/96T1800/2800进口分装F28120狗表皮生长因子(Canine EGF)ELISA48T/9 ...

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选择3~5轮后，随机挑取克隆加入96孔微量滴定板中 并诱导表达可溶性scFv.然后，在包被有相关抗原的96孔微量滴定板中，用ELISA分析含有可溶性scFv的细菌上清。此过程可以识别出那些结合了抗原的scFv.但即便采用上述严谨性选择后，ELISA阳性克隆中仅有部分克隆的亲和力比野生型高。ELISA信号强度不能很好地显示哪个克隆具有较低的Ka值，而且更为普 遍的是它与scFv表达水平相关。因此， ...

● PCR试剂和设备● cFv基因文库单链模板DNA制备自pHENl中的天然scFv文库（10ng/u1）● Gelleclean试剂盒（Qbiogene）● Wtzard PCR纯化试剂盒（ProlneSa）● RJHl/2Xho引物： 5'-GGC ACC CTG GTC ACC GTC TCG AGT GGT GGA-3'● RJH3Xho引物： 5'-GGG ACA ATG ...

● PCR试剂和设备● 含有起始scFV基因的载体pHENl单链模板DNA（50ng/ul）● Geneclean试剂盒● Wlzard PCR纯化试剂盒● scFvJHL2XhoFOR引物：5'-GAG TCA TTC TCG TCT CGA GAC GGT GAC CAG GGT GCC-3'● scFvJH3XhoFOR引物： 5-GAG TCA TTC TCG TCT CGA ...

Purpose and backgroundsAbout nuclide...35-SDecay: (b-ray 0.167 MeV)Half life: 87.5 daysThick acrylic shield can block most of the emission. Because of the low energy it is impossible todetect spillage ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

PurposeMaterialsAntibody 7E3 2L sup grown in flasks frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 10 ...

PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid quantitative multiparameter analyses on singl ...

PurposeThis is a guideline for animal experiment. ProcedureAnimal Protocol An active animal protocol is needed for any experiment involving animals. If the experiment is not covered by the existing an ...