抗体抗原实验

Live Cell ELISA ProtocolAuthor: Nanci DonackiSource: Contributed by Nanci Donacki ReagentsDMEM:10% FBSDMEM:10% FBS:1% HEPES (DFH)10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g ...

Peptide Inhibition ELISA ProtocolAuthor: Nanci DonackiSource: Contributed by Nanci DonackiReagents PBSPBS:0.1% BSA:0.05% Tween 20 (PBT)PBS:2% BSA10X Substrate Buffer pH 6.036.6 g Citric Acid mon ...

Periodate Treatment of Fixed Cell PlatesAuthor: Nanci DonackiSource: Contributed by Nanci DonackiReagentsSodium Borohydride (NaBH4) - 1 mg/mlCarbonate:Bicarbonate Buffer pH 9.6 Sodium Periodate (NaIO4 ...

FIXATION WITH FORMALDEHYDE-ACETONEMaterials1. Dulbecco's PBS 37oC. 2. Fixation buffer i.e. PBS PHEM or modified Hanks ("cytoskeleton buffer") 1.33x. 3. Formaldehyde 16% EM Sciences. Store at room temp ...

Fixation with GlutaraldehydeMaterials vol (for 500 ml) of stock mg 1. 137 mM NaCl 34.25 ml 2 M 5 mM ...

Embedding in Resins for ImmunocytochemistryBiological specimens are embedded in resin so that they can be thin sectioned. Many different resin formulas are currently available and all have been used f ...

Freeze Substitution How to prepare samples using freeze-substitution without an expensive machine.This method has been used successfully in our laboratory since 1994. The protocol assumes all users wi ...

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detectionSections of paraformaldehyde fixed OCT-embedded vascular tissue are secti ...

MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems 'AF' or 'BAF' series) or selected monoclonal antibodies.Secondary An ...

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Vector Red DetectionJosiah N. Wilcox and Romesh R. Subramanian - Emory University - September 20 1993 Sections ...

Immunostaining Protocol (now with demo videos/stills)We recommend that you first download and read the PDF file containing the step-by-step protocol and solution recipes. Using that as your guide you ...

Immunostaining of Cells Adherent to CoverslipsProcedure:1) Immerse 18 mm2 glass coverslips in EtOH. In the tissue culture hood individually pull out and carefully flame to sterilize. Allow to cool the ...

Introduction to ImmunocytochemistryImmunocytochemistry is the name given to methods that use antibodies to detect the location of proteins within cells. The antibodies bind specifically to the protein ...

General remarks:Labeled sections have to be fixed to avoid loss of label during the enhancement procedure and thoroughly washed with bi-distilled water to remove ions (especially chloride ions) which ...

Immunofluorescence on ultrathin resin sectionsThis protocol is taken from the 1999 FEBS course manual and was contributed by Heinz Schwarz.General remarks:Ultrathin methacrylate-(e.g.Lowicryl HM20 K4M ...

Antibody cleanupIn order to decrease the amount of nonspecific staining it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixtu ...

Immunocytochemistry: Common problems and some answers.Immunocytochemistry at the ultrastructural level (TEM)Results obtained from immunocytochemical labeling can often differ from what was expected. S ...

Labeling Muscle Actin with 5-iodoacetamidofluoresceinDay 0 and 1Materials1. 0.5 mM ATP 0.2 mM CaCl2 2 mM Tris-HCl pH 8.0 at 4oC 250 ml for day 0. 2. 100 mM KCl 100 mM boric acid pH 8.4 at 4oC 10 ml. 3 ...

Preparation of Colloidal Gold Conjugates.Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years colloidal gold particles have b ...

A semi-permanent mounting medium for immunofluorescence microscopyMaterials6gm glycerol 2.4gm mowiol 6ml distilled water 12ml Tris buffer 0.2M at pH 8.5 MethodWeigh out the glycerol in a 50ml plastic ...