基因表达差异显示实验

The human herpesviruses represent excellent candidate viruses for several types of gene vector applications. As a class, they are large DNA viruses with the potential to accommodate large or multiple transgene cassettes, and they have evolved to persist in a lifelong nonintegrated lat ...

The adeno-associated virus 2 (AAV2) is known to possess a broad host-range that transcends the species barrier (1). The broad host-range and nonpathogenic nature of AAV, coupled with its site-specificity and stable integration of the pro viral genome, have led to the development of recombina ...

The number of published studies on transduction of tumor cells in vivo using recombinant adeno-associated virus (rAAV) vectors is very limited compared with those that have been published on targeting normal cells. A major reason for this can be attributed to the biology of the vector itself. AA ...

The efficient transduction of postmitotic cells by adeno-associated virus (AAV) makes it an excellent vector to deliver marker, functional, or therapeutic genes to the mammalian brain. An attractive feature of AAV is that all the viral-coding sequences are removed when engineering the r ...

There are a large number of cardiovascular diseases that could be treated by myocardial gene transfer (1,2). These include congestive heart failure, ischemic heart disease, and cardiomyopathy. In addition to its potential for treatment of disease, myocardial gene transfer is useful for ...

The ability of adeno-associated viral (AAV) vectors to promote persistent gene expression in nondividing cells in multiple somatic tissues of animals (1–4) makes them excellent tools for gene transfer. One tissue of interest for gene transfer is the lung epithelium, which is afflicted in cy ...

The liver is a frequent target of gene-transfer experiments, because of its central role in many metabolic and synthetic pathways. For applications where prolonged expression of genes in the liver is required, adeno-associated virus (AAV) has proven to be an effective tool for in vivo gene tran ...

Adeno-associated viral (AAV) vectors are derived from a nonpathogenic, replication-deficient virus with a small (∼4.7-kb) single-stranded DNA genome. AAV vectors are devoid of viral-coding sequences and may efficiently transfer genes to nondividing cells such as muscle fibers or h ...

Adeno-associated virus (AAV) gene delivery vectors are being investigated as vehicles for gene therapy for a wide variety of hereditary and acquired human diseases. AAV’s inability to self-propagate, ability to be maintained as an episome in the transduced cell, and relatively innocuo ...

The use of adeno-associated virus (AAV) as a gene transfer vector has been steadily increasing over the past several years. A AV vectors have been particularly useful for applications where sustained gene expression is required. Prolonged in vivo expression following A AV treatment has been ...

Dendritic cells (DCs) are “professional” antigen-presenting cells (APCs) that are uniquely capable of activating and instructing a naive immune system to mount a specific cellular and humoral response. Recognition of this crucial function makes the development of technologies f ...

Cell transduction in vitro is only the first step toward proving that a gene-therapy vector can be useful to treat tumors. However, tumor targeting in vivo is now the milestone for gene therapy to succeed against disseminated cancer. Therefore, most valuable information is obtained from stud ...

Recombinant adenoviral (Ad) vectors are derived from human adenoviruses: nonenveloped, encapsidated linear, double-stranded DNA viruses that commonly cause respiratory and gastrointestinal infections. Forty-three different human adenovirus serotypes have been c ...

The development and characterization of protein microarrays fabricated on nanoengineered three-dimensional polyelectrolyte thin films (PET) deposited on glass slide by consecutive adsorption of polyelectrolytes in solutions through self-assembly process were de ...

The ability to perform microarray-based immunoassays without the need for wells or other fluid barriers were demonstrated. Both contact and noncontact microarray printing technology is used to prepare spotted arrays of analyte binding sites, as well as, to deliver samples, secondary ...

We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using a microRNA detection chip, (2) protein binding and function analysis us ...

Among vertebrates, the mammalian systems that are frequently used to investigate questions related to human health have gained the most benefit from microarray technology to date. However, it is clear that biological investigations and the generalized conclusions drawn from them, c ...

A new strain of coronavirus has caused an outbreak of severe acute respiratory syndrome (SARS) from 2002 to 2003 resulting in 774 deaths worldwide. By protein chip array profiling technology, a number of serum biomarkers that might be useful in monitoring the clinical course of SARS patients we ...

Affinity mass spectrometry is a proteomics approach for selectively isolating target proteins from complex biological fluids for mass spectrometric analysis. When executed in high throughput mode through affinity pipets, the resulting volumetric mass spectrometry arrays ...

Systems biology has enjoyed explosive growth in both the number of people participating in this area of research and the number of publications on the topic. The field of systems biology encompasses the in silico analysis of high-throughput data as provided by DNA or protein microarrays. Along ...