基因表达差异显示实验

The prediction of RNA secondary structure differs significantly from that of protein secondary structure. The latter is based on crystallographic data, and by using a variety of empiric or semiempiric parameters, a prediction is achieved. RNA secondary structure prediction, on the ot ...

Most of the programs presented in the earlier chapters, including those in Chapter 14, work on both peptide and nucleotide sequences. There are a few GCG programs, however, that require protein sequences. Method 1, pattern matching, describes how to identify features of unknown sequences bas ...

Translation of DNA sequences can be simply trivial reformatting, e.g., transcribe one file format into another, or use Us instead of Ts. and so forth. These utilities for “translation” are by no means unimportant and are, therefore, presented in Method 1.

Pattern recognition in biological data is difficult to achieve. On the one hand, it is simple trying to find information based on identity of patterns described in a search string. Method 1 and 2 describe such a pattern search for text strings. More sophisticated searches use a pattern definition la ...

Searches in databases require efficiency and speed. This cannot be achieved by using the same methods as described in the previous chapters on sequence-comparison. It would take much too long to calculate alignment path matrices between the database sequence and the query sequence. Howev ...

DNA sequencing methodology was developed in the late 1970s (1,2) and has become one of the most widely used techniques in molecular biology. The amount of DNA sequencing performed worldwide has increased exponentially each year and this trend will almost certainly continue for the next few ye ...

光遗传学技术是一种结合遗传学与光学技术，在复杂如自由活动个体的生物系统中实现定点的、快速的控制某一精确定义的生物学过程的技术。通过引入光敏感蛋白的受体或通道蛋白至特定组织特定细胞中，并经特定参数的光信号控制，光遗传学技术能够关闭或激活某一类细胞的生物学功能，从而实现在细胞、环路、器官和个体等多个层面研究该细胞及其环路的生物学机制与功能性意义。 在神经科学的研究中，一个重要的问题是脑 ...

研究人员发明一种新方法，有助于确定变异在多发性硬化症中所发挥作用的程度，新成果发明在5月在线出版的《自然―结构和分子生物学》期刊上。 通过人类基因组的研究，科学家们能够确定变异与特定疾病的关系，但他们至今仍难以将许多变异与某个特定的基因联系起来，从而导致了遗传学研究与疾病治疗的分离，但是，如果不能确定靶向基因，遗传性疾病是难以治疗的。 Fernando Casares J ...

基因敲除，过表达和异常表达可被用来研究在发育过程中某个基因发挥的作用。在此我们介绍一种通过显微注射mRNA和吗啉代反义寡核苷酸实现过表达EGFP并敲除pkd2的方法。

借助显微注射技术可导入mRNA或吗啉代至斑马鱼胚胎。显微注射法效率高，速度快，而且每小时可注射数百的胚胎。本视频对显微注射涉及到的所有步骤作了详细说明。

本视频介绍利用绿色荧光蛋白（GFP）基因连续监测相同组织基因表达的方法。该绿色荧光蛋白基因可以由不同的启动子调节控制，利用粒子轰击技术将GFP基因导入菜豆子叶组织后，将子叶放至一个自动图像采集系统采集图像， 然后根据收集到的图像分析植物组织的基因表达情况。

生物发光和化学发光（BL/CL）用于基因表达 体内基因表达模式方面的研究。在药物研发领域，科学家通过可发光转基因动物（例如：大鼠或小鼠基因组中整合了修饰的内源基因和BL报告基因）作为人类疾病的模式动物进行目标确认的研究。 例如：目前已经建立了一个转基因小鼠模型，可以监测编码人细胞色素P450 3A4（CYP3A4）基因的体内转录调控，人细胞色素P450 3A4在药物代谢方面起着重要作用。 ...

原核生物基因表达系统：如大肠杆菌表达系统、芽孢杆菌表达系统、链霉菌表达系统、蓝藻表达系统等。

原核生物基因表达系统：如大肠杆菌表达系统、芽孢杆菌表达系统、链霉菌表达系统、蓝藻表达系统等。

原核生物基因表达系统：如大肠杆菌表达系统、芽孢杆菌表达系统、链霉菌表达系统、蓝藻表达系统等。

原核生物基因表达系统：如大肠杆菌表达系统、芽孢杆菌表达系统、链霉菌表达系统、蓝藻表达系统等

科学家提出，让通过虱子传播的莱姆病病源体——莱姆病螺旋体（Borrelia burgdorferi）的一个基因不再活动可以阻止这种疾病的传播。此前的研究表明莱姆病螺旋体的bba64基因在进食的虱子体内表达，然而这种基因的功能尚不为人所知。Robert Gilmore, Jr及其同事为小鼠注射了一种莱姆病螺旋体菌株，这种修改后的菌株的bba64基因被关闭了，然后让虱子以小鼠为食并获得了这种细菌。

采用OG05 差异显示技术，分析水稻稻瘟病抗源材料“ 地谷”叶片受稻瘟病菌侵染前后的基因的表达差异，获得87个差异片段。对这87个差异片段进行了回收、重扩增与克隆，并对其中的81个片段进行了杂交鉴定。斑点杂交结果证实其中6个片段受稻瘟病菌诱导表达。进一步克隆测序并进行数据库比对分析表明其中一个与水稻4号染色体中一推测的苹果酸合成酶高度同源，一个与水稻11号染色体上的RPR1 ...

All living organisms have thousands to tens of thousands of unique genes encoded in their genome of which only a small fraction perhaps 15% are expressed in any individual cell. Therefore it is the te ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...