转基因实验

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完整的实验方法请点击下面的链接下载： ...

The manipulation of embryonic stem (ES) cells to generate targeted mutations via homologous recombination has proved an invaluable resource for researchers from fields as diverse as embryology immunol ...

10 ul reactions amounts per reaction Make MM for 1 extra reaction every 6 ...

Introduction PCR protocols to detect rare deletions in DNA from complex populations of mutagenized worms typically depend on a large size differential between the wild-type product and the deletion p ...

Overview Our protocol for generating deletion mutations is based on the procedures of Bob Barstead and Gary Moulder at the Oklahome Medical Research Foundation in Oklahoma City. The procedure is to m ...

1、 选取7~8周龄雌性小鼠，阴道口封闭，作为供体，下午3:00左右，每只小鼠腹腔注射PMSG（10 IU）。 2、 47~48小时后，每只小鼠腹腔注射HCG（0.8 IU），并与正常公鼠合笼；另取数只适龄母鼠（2月龄以上）作为受体，阴道口潮红，与结扎公鼠合笼。 3、第二天上午9:00前观察供体、受体，有精栓者拿出备用。受体笼拿出作好隔离措施。 4、10:30左右，断颈处死供体，手术取出整个 ...

1. Introduction. This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene t ...

Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Aust ...

Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra ...

Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (284 ...

1-cell embryo transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - those that have been damaged during t ...

The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host ...

General Comments Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µ ...

This is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately I can't attribute much of the development work since I was general ...

2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2 ...

The number of infertile couples has increased in recent years and is now estimated at 15–20% of all couples worldwide. Since its introduction into reproductive medicine in 1978 in vitro fertili ...

The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor ...

Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl2 up to 100 ml with Q store at room temperature 1M KPO4 pH 7.5 250 ml 1M KH2PO4 (monobasic) 68.05 g up ...