Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouth Gavage - - into the stomach via a feeding needle Parenteral Administration:General Consid ...
Materials Needed: Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip 2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samplesRack to hold tubesFine tip marker to label tu ...
The ear veinsThe marginal ear veins are the only veins that are easily visible on pigs of any size. Usually there are three prominent veins. The lateral or central vein is usually the largest of these ...
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg in PBS dose of 150 mg/kg) that is allowed to distribute in awake animals for about 5-15 minutes. The mice ...
Preparation of Islets for Transplant (Tx)Under an inverted microscope hand-pick islets using a P200 pipetman and straight pipet tip from the cultured islets in a 100mm plate. Count islets 100 at a tim ...
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimenta ...
You need to buy glass slides with frosted sandblasted ends (Fisher Scientific Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges o ...
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inha ...
if mouse is found dead if mouse is a newborn or If mouse is alive or moribund and above one week of age Routine dissection Formalin fixation Storage of smears and slides Tissue embedding in paraffin ...
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help ensure optimal results for aseptic survival surgery ...
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guide for the Care and Use of Laboratory Animals all survi ...
Isolation of bone marrow (contributed by Chris Jackson (chris.jackson@bris.ac.uk)) A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the femurs cutting the bones cl ...
This is prepared as a 1.2% solution and used in mice at a dosage rate of 0.2 ml/10grams body weight which approximates 240 mg/kg. This compound is subject to photodegradation and should be stored at 4 ...
The method we use is based on work of Dr. Hazel Cheng at the University of Toronto and works for both colon and the small intestine.First we excise the whole colon from cecum to rectum. This is easily ...
Prior to the initiation of experiment:Obtain IACUC approval Sterilize surgical instruments and Q-tips I. Harvest Donor TissueEuthanize donor mouse (in accordance with institution's SOP) Wet hair back ...
PROTOCOLGeneral anesthesiainject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation of ...
What is Anesthesia?Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief) amnesia (loss of memory) and immobilization. The drugs ...
1) Transfer to a 24 wells plate the desired colonies.2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml ofDME (met- cys-).3) Add 3l of Met 35S Cys 35S trans label.4) I ...
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (PPE). Many of them are common sense and apply to all la ...
Although many protocols for tubulin preparation are available the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains and shoul ...
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