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Purpose To describe aseptic techniques used by Cell Culture specialists in handling products from and/or mammalian cells. Safety Protect eyes mucous membranes open cuts and wounds from contact with bi ...
The use of aseptic technique is essential for avoiding the production of infection whilst undertaking tissue culture activities.Many activities take place within a laminar flow hood either Class I (us ...
You will need:- 12g Potassium Permanganate 6g Crushed paraformaldehyde 1) Set the hood so that it can vent outside. 2) Mix the two chemicals above together (in a jam/coffee jar so it can be thrown awa ...
Sterile TechniqueGood sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers t ...
Measurement of DNA concentration using PanVera fluorescence assaySteve HahnLast updated 9/30/98This Dye measures DNA concentration using a fluorescent dye which binds only double stranded DNA. The flu ...
1. Dilute radioactive sample to a 100 ml volume2. Spot 5 ml of the sample onto the center of a 2.4 cm Whatman GF/C glass-fiber disc.3. Mix 5 ml of the sample with 100 ml Salmon sperm DNA (50 mg in 20 ...
点击浏览该文件相关产品链接：试剂库 核酸检测 核酸定量检测试剂库 PCR/RT-PCR/qPCR 定量PCR试剂仪器库 分子生物实验仪器紫外设备仪器库 影像系统凝胶成像系统仪器库 测读系统分光光度计 ...
环境试验作为可靠性试验的一种类型已经发展成为一种预测产品使用环境是如何影响产品的性能和 以下内容需要登录后才能完全查看，请您登录马上登陆| 企业会员 ...
（一）小鼠用右手提起小鼠尾部，放在实验台上，当其向前爬行时，左手抓住两耳及头颈部皮肤，再置小鼠于左手心，拉直四肢并用手指夹住肢体固定。右手可行注射或其它操作。小鼠固定器及固定器固定法（二）大鼠右手轻轻抓住大鼠尾部向后轻拉，左手抓紧鼠两耳及头颈部皮肤，并将其固定在左手中，右手可行注射或其它操作。大鼠固定器及固定器固定法（三）兔1、正确的抓取兔的方法如下：说明：1、可伤两肾；2、可造成皮下出血；3、 ...
对于像黑色、棕色小鼠以及裸鼠，我们不能单纯用常规方法编号，因此对于这类动物，可以采用一些特殊的编号方法。一、采取耳朵打孔编号1号双耳无孔； 2号左耳打孔； 3号右耳打孔； 4号双耳打孔； 5号左耳双孔。二、耳缺法左前缺-1号； 左后缺-2号； 右前缺-3号； 右后缺-4号； 1+2――5号； 3+4――6号； 1+3――7号； 2+4――8号。耳缺法隔着笼子也好观察。裸鼠耳朵 ...
捉拿和固定是动物实验操作技术中最基本最简单而又很重要的一项基本功。 以下内容需要登录后才能完全查看，请您登录马上登陆| 企业会员注册 ...
分子生物学常用溶液配制一、分子生物学常用贮存液的配制1．30%丙烯酰胺溶液【配制方法】将29g丙烯酰胺和1g N，N’-亚甲双丙烯酰胺溶于总体积为60ml的水中。加热至37℃溶解之，补加水至终体积为100ml。用滤器（0.45μm孔径）过滤除菌，查证该溶液的pH值应不大于7.0，置棕色瓶中保存于室温。【注意】丙烯酰胺具有很强的神经毒性并可以通过皮肤吸收，其作用具累积性。称量丙烯酰胺和亚甲双丙烯酰胺 ...
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has great potential for causing pain or distress to an ...
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic procedures. This includes the use of surgical gloves ma ...
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictates PROCESSING AND STORAGE OF TISSUES:1. BrainRemo ...
ProcedureAfter deep ether anesthesia dissect the rat’s liver (Wistar albino rats 200 � 250 g) by cutting on the ventral side. Fix 2 � 3 mm. of the liver tissue in neutral buffered formalin (10% formal ...
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