实验仪器使用技术

Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help ensure optimal results for aseptic survival surgery ...

Isolation of bone marrow (contributed by Chris Jackson (chris.jackson@bris.ac.uk)) A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the femurs cutting the bones cl ...

The method we use is based on work of Dr. Hazel Cheng at the University of Toronto and works for both colon and the small intestine.First we excise the whole colon from cecum to rectum. This is easily ...

This is prepared as a 1.2% solution and used in mice at a dosage rate of 0.2 ml/10grams body weight which approximates 240 mg/kg. This compound is subject to photodegradation and should be stored at 4 ...

PROTOCOLGeneral anesthesiainject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation of ...

What is Anesthesia?Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief) amnesia (loss of memory) and immobilization. The drugs ...

Preparation of tumor cells Grow cells in complete medium and exclude any contamination When cells are 70-80% confluent 3-4 hrs before harvesting replace medium with fresh medium to remove dead and det ...

1) Determine the number of cells for injection (ie 5´106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent plate of 100mm2 will yield at least 2injections at ...

Isolation of T4 cells from xenograft tumorsPut mouse down Wash skin by dipping mice into ETOH Remove tumor and put into 5ml PBS containing Fungizone and Penstrep Swirl around to rinse tumor and pour ...

For the establishment of solid tumour in nude mice tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile plastic pipettes and flasks are used. Cultures are ...

Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use add 50 ml acetic acid to 10% final concentration. 4) Stain for 2 ...

1) Transfer to a 24 wells plate the desired colonies.2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml ofDME (met- cys-).3) Add 3l of Met 35S Cys 35S trans label.4) I ...

How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum at -70º C as it does not prolong the shelf life o ...

Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine add: 165.0 ml fetal bovine serum heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (10 ...

MS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock soluti ...

These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or flash frozen rat liver PBS Homogenization Buffer Homogeniz ...

I. Solutions: A. Ca and Mg free Phosphate Buffered Saline (PBS) solution buffered with 0.02M Hepes. pH=7.4 B. Ca and Mg free PBS buffered with 0.02M Hepes and 1mM EDTA. pH=7.4 C. PBS with ...

Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described he ...

I. Useful Values 1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100000; in reality it is ~110000 but almost all tubulin labs use this convenient conversion relationship) 1 µm of a m ...

Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted). Make up the developer and the fixer and place in the darkroom ready for later (only if developing ...