实验仪器使用技术

Embedding in a plastic often supplies the support necessary to successfully section various materials samples especially porousand inhomogeous samples. GMA has been used to embed and section polymer r ...

Procedure: Oxidize sections in 0.4% periodic acid for 30 minutes at 57°C. Wash in running tap water or sodium sulfite solution (to avoid a general pink background). Rinse in three times in distilled w ...

SectioningSectioning is best done with a rotary or sledge microtome such as the JB-4 Microtome. The features to look for are retraction of the specimen on the return stroke and motorized motion of the ...

Procedure: Dry the sections for 2 hours at 37°C on a slide warmer. This must be done whether the slides have been stored at 4°C for some time or just collected. Pretreat the sections with trypsin at 3 ...

Day 1 Step 1: Read and understand the Material Safety Data Sheets associated with each of the chemicals used in this procedure. Use of rubber gloves lab coat and safety glasses is mandatory.Step 2: On ...

IntroductionSpecimens that are to be examined in the transmission electron microscope (TEM) usually have to be thin dry and contain contrast (usually from a heavy metal stain. One of the easiest ways ...

Basic Processing Protocol for Tissue Culture Cells Grown in Plastic Dishes. This method can easily be applied most biological samples without problem. 1). Wash cells with PBS twice. Remove the PBS and ...

Basic Processing Protocol for Tissue Culture Cells Grown in Plastic Dishes. This method can easily be applied most biological samples without problem. 1). Wash cells with PBS twice. Remove the PBS and ...

A few notes on fixation:The usual fixative for paraffin embedded tissues is neutral buffered formalin (NBF). This is equivalent to 4% paraformaldehyde in a buffered solution plus a preservative (metha ...

Deparafinization: The paraffin is removed from the section with xylene. If the tissues are to be stained with an aqueuous solution then the slides must rehydrated in graded ethanol baths. Unless a tim ...

ReagentsStains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fre ...

ReagentsStains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fre ...

Syringes 1 mL with 36G needlesBrdU: 3-10 mg/mL in PBSFixative: Methyl Carnoy's or 4% paraformaldehyde in PBSParaffin processing materialsOrganic solvents: Xylene EtOH MeOH0.3% H2O2: 2 mL 30% H2O2 i ...

MaterialsFixative2% Paraformaldehyde (in PBS)Staining solution5 mM potassium ferricyanide 5 mM potassium ferrocyanide 2 mM MgCI 0.01% sodium deoxycholate0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock4 ...

MaterialsCold Acetone (-20ºC) in Coplin jarsPBS BenchKote paper plastic lidsAnti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC)Anti-rat-digoxigenin diluent: 5% Milk po ...

MaterialsAnti-digoxigenin-AP conjugate (Roche #11093274910 150U/200 uL $168)Levamisole (Sigma #T1512 2 g $10) (blocks endogenous phosphatase activity)BCIP (Roche #11383221001 3 mL $49)NBT (Roche #113 ...

MaterialsCold Acetone (-20ºC) in Coplin jarsPBS BenchKote paper plastic lidsAnti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC)Anti-rat-digoxigenin diluent: 5% Milk po ...

MaterialsAnti-digoxigenin-AP conjugate (Roche #11093274910 150U/200 uL $168)Levamisole (Sigma #T1512 2 g $10) (blocks endogenous phosphatase activity)BCIP (Roche #11383221001 3 mL $49)NBT (Roche #113 ...

Materials24 well dishesMillicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectinCulture mediumPBSFixative: 4% paraformaldehyde in PBS (pH 7.6)Rabbit anti-GFP (Molecular Probes A-11122) ...

测微尺的使用:测微尺分目镜测微尺和镜台测微尺，两尺配合使用。目镜测微尺是一块圆形玻璃，中心刻有一尺，长5~10mm，分成50~100格。每个所代表的实际长度因不同物镜的放大率和不同镜筒长度而异。镜台测微尺是在一块载玻片的中央，用树胶封固一圆形的侧微尺，长1~2mm，分成100或200格。每格实际长度为0.01mm（10μm）。当用目镜测微尺来测量细胞的大小时，必须先用镜台测微尺核实目镜测微尺每 ...