实验仪器使用技术

A few cell types are thin enough to be viewed directly in a microscope (algae protozoa blood tissue cultures) but most tissues (kidney liver brain) are too thick to allow light to be transmitted throu ...

Microwave Processing Techniques for Microscopy From Energy Beam Sciences Inc. Agawam MA How does a microwave oven work?How does a laboratory microwave processor differ from a kitchen microwave oven?Sa ...

BIELSCHOWSKY METHOD SECTIONS8micron paraffin wax sections.SOLUTIONS1. Silver A - 20% silver nitrate2. Reducer (Make fresh) Formaldehyde 50mlAbsolute Alcohol 200ml Distilled water 750ml 3. Silver B20% ...

For DNA analysis:Special LCM processing schedule is followed.The water bath is cleaned using RNAse Zap™ rinsed thoroughly with triple distilled water. The water bath is filled with triple distilled wa ...

3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES Wash glass slides in detergent for 30 minutes.Wash glass slides in running tap water for 30 minutes.Wash glass slides in distilled water 2x5 minutes.Wash gl ...

Special Stains Using the MicrowaveJoyce Moore HT/HTL (ASCP) Technical SpecialistJefferson Regional Medical Center Histo-Path Laboratories1515 West 42nd Avenue . Pine Bluff AR 71603(501) 541-7544 A Lit ...

Working Procedures in Microwave Histologyby Vincent R. Klump Jr. HT (ASCP) Histology Services East Haven CT note: Mr. Klump was instrumental in the development of a standardized consistent reproducibl ...

4% PARAFORMALDEHYDE 4% PARAFORMALDEHYDE/PBS 1. Measure 100ml Phosphate Buffered Saline (PBS) into a measuring cylinder. Pour into the conical flask containing 4g of paraformaldehyde. Cover with parafi ...

10% FORMAL SALINE Wear appropriate protective clothing eg. laboratory coat gloves and goggles.Weigh out 900g of sodium chloride and dissolve in warm water.Unscrew cap on porthole of formalin mixing ta ...

Slide Preparation for Manual Microdissection(for Subsequent DNA RNA and Protein Analysis)These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators m ...

Fixation: Confers chemical stability on the tissue Hardens the tissue (helps further handling) Halts enzyme autolysis Halts bacterial putrefaction May enhance later staining techniques Introduces a 'c ...

I. Preparation LCM and RNA/DNA extraction of Frozen Tissue Sections A. Embedding 1. Place an empty labeled cryomold on dry ice for 1 min. It should remain on dry ice during the entire embedding proced ...

Slide Preparation for Manual Microdissection(for Subsequent DNA RNA and Protein Analysis)These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators m ...

ALKALINE CONGO RED TECHNIQUE SECTIONS4µm paraffin wax sectionsSOLUTIONS1. STOCK ALCOHOLIC SALT SOLUTION--Saturated sodium chloride in 80% alcohol2. WORKING SOLUTION--Stock solution (1) 50cm3 --1% Sodi ...

Processing of Microdissected Tissue for Molecular AnalysisDNA-based Studies More than 10000 CellsLess than 10000 CellsFormalin-fixed Paraffin-embedded TissueOther FixativesTimingThese methods were suc ...

Processing of Microdissected Tissue for Molecular AnalysisDNA-based Studies More than 10000 CellsLess than 10000 CellsFormalin-fixed Paraffin-embedded TissueOther FixativesTimingThese methods were suc ...

Microwave Tissue ProcessingFrom Donna Willis Harris Methodist Hospital Ft. Worth TexasPURPOSE: To provide rapid tissue processing that will decrease turn-around-time.PRINCIPLE: Microwave exposure is u ...

Microwave Processing Schedules for BiopsiesProcedure IIIUsed by Denise Hardy ARUP Laboratories Salt Lake City UT. Pre-fix room temperature 1 hour. Microwave in reagent alcohol 67°C 5 minutes. Microwa ...

Incubate sections in the incubating medium at 37°C for 5 - 12 hours. Long incubation periods are needed to get significantly visible reaction product. Wash in distilled water for 2 minutes. Countersta ...

Using the Components The basis solution Technovit 9100 NEW can be used in stabilized or destabilized form. The use of destabilized Technovit 9100 NEW basis solution guarantees results for all immunohi ...