Peritoneal metastasis is the most important prognostic factor for gastric and ovarian cancer patients. The protocol in this chapter presents in vivo imaging procedures capable of examining the development of peritoneal metastasis from the micrometastasis stage to the advanced s ...
Pancreatic cancer is one of the most aggressive human malignancies. One of the leading causes of pancreatic cancer death is metastasis. The early stages of tumor progression and micrometastasis formation have been difficult to analyze and have been hampered by the inability to identify sm ...
In vivo angiogenesis assays provide more physiologically relevant information about tumor vascularization than in vitro studies because they take the complex interactions among cancer cells, endothelial cells, mural cells, and tumor stroma into consideration. Traditional ...
Tumor-specific replication-competent viruses represent a novel approach for the treatment of neoplastic disease. These vectors can be used to directly label tumor cells in vivo, as they are designed to selectively replicate within such cells. To target cancer cells, there is a need for tiss ...
Methods for the construction of electrochemical composite biosensors using gold nanoparticles and Teflon as nonconducting-binding material are described in detail. The advantages of the incorporation of gold nanoparticles to the composite electrode matrices are highlig ...
A model immunosensor based on a labeling method using gold nanoparticles (AuNPs) and electrochemical detection is developed. Microparamagnetic beads (MB) as primary antibody immobilization platforms and AuNPs modified with a secondary antibody as high sensible electroche ...
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by im ...
Microcantilevers based-biosensors are a new label-free technique that allows the direct detection of biomolecular interactions in a label-less way and with great accuracy by translating the biointeraction into a nanomechanical motion. Low cost and reliable standard silicon te ...
Piezoelectric quartz crystals serve as resonator-based transducers for direct and real-time monitoring of immunoaffinity interactions. The measuring system is briefly characterized; several examples for immobilization of antibodies are recommended. The piezoelec ...
Aptamers can be considered as a valid alternative to antibodies or other biomimetic receptors for the development of biosensors and other analytical methods. The production of aptamers is commonly performed by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) p ...
Piezoelectric sensors have become a versatile tool in biosensorics to study protein—protein and protein—small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition el ...
The method of fabrication of a prototype electrochemical immunosensor for estradiol (E2) is described. Methodologies are also given for colorimetric assays, which can be used to verify and optimize reagent performance, prior to their use in the electrochemical immunoassay: these in ...
An electrochemical DNA biosensor is a device that utilizes the inherent ability of two complementary strands of nucleic acids to form a double helix. The specificity of this reaction, namely hybridization, is used in the detection of target DNA sequences with a view toward developing point-o ...
In recent years, electrochemical sensors and biosensors are becoming an accepted part of analytical chemistry since they satisfy the expanding need for rapid and reliable measurements. An area in which electrochemical biosensors perhaps show the greatest diversity and potential ...
The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibo ...
A novel, rapid, and sensitive protocol for the electrochemical detection of DNA hybridization that take the advantage of a magnetic separation/mixing process and the use of monomaleimido-gold nanoparticles of 1.4 nm diameter as label is presented. A sandwich-type assay is formed in this p ...
Clinical and environmental analyses frequently necessitate rapid, simple, and inexpensive point-of-care or field tests. These semiquantitative tests may be later followed up by confirmatory laboratory-based assays, but can provide an initial scenario assessment importa ...
Principles and characteristics of lateral flow strip assays are reviewed. Recent technology developments permit the use of inexpensive electronic readers for interrogating lateral flow strip test results, thus avoiding the inevitable variation and subjectivity of visual in ...
In recent years, immunochromatographic lateral flow test strips are used as a popular diagnostic tool. There are two formats (noncompetitive and competitive) in gold-based immunoassay. Noncompetitive gold-based immunoassay also called sandwich assay is applied for the detect ...
Though relatively unexploited in biosensor applications, phage display technology can provide versatile recognition scaffolds for detection of cancer markers and other analytes. This chapter details protocols for covalent attachment of viruses directly to electrodes for ...
关于丁香通
公司信息
个人用户
企业机构
