实验仪器使用技术

The introduction of PET(-CT) has brought about a major paradigm shift in the management of thyroid carcinoma, especially from the diagnostic standpoint. From the viewpoint of patient management, the areas where it has made significant impact include the following: (1) the detection of dise ...

Sarcomas are a diverse group of malignancies originating in the connective tissue. The approach of a patient with a mass suspect for sarcoma starts with performing a biopsy to obtain tissue for evaluation by pathology. The main role of the current imaging modalities, in general, is to recognize pa ...

Diagnostic imaging modalities utilized in the care of cancer patients must fulfill several requirements: they must diagnose and characterize tumors with high accuracy, must reliably stage and restage the disease, and should allow for monitoring the effects of therapeutic interve ...

The clinical problems raised in patients presenting with all forms of gynecological malignancy are currently addressed using conventional cross-sectional imaging, usually MRI. In general, F-18 FDG PET-CT has not been shown to have a clinical role in any of these cancers at presentation, a ...

Neuroendocrine tumors (NET) have several distinct pathophysiological features that can be addressed by specific radiolabeled probes. An overview on the different radiopharmaceuticals that have been developed for positron emission tomography (PET) of NET are presented. The f ...

This chapter discusses the value of FDG-PET and combined FDG-PET/CT in staging and follow-up of melanoma patients. For melanoma patients, the presence or absence of regional lymph node metastases is one of the most important prognostic factors; the recent development of sentinel lymph node ...

This chapter aims at giving a quick but precise introduction of the atomic force microscope from the working principle point of view. It is intended to provide a useful starting point to those who first approach the instrument giving a general sketch of the working principles and technical imple ...

This chapter is introductory to the measurements: it explains different measurement techniques both for imaging and for force spectroscopy, on which most of the AFM experiments rely. It gives a general overview of the different techniques and of the output expected from the instrument; the ...

Atomic force microscopy (AFM) measurements could be affected by different kinds of artifacts; some of them derive from the improper use of the instrument and can be avoided by setting the correct experimental parameters and conditions. In other cases, distortions of the images acquired by AFM ...

This chapter describes methods for isolating and imaging metabolically and toxicologically challenged mitochondria with atomic force microscopy. Mitochondria were isolated from rat dorsal root ganglia or brain and exposed to glucose or dinitrobenzene (DNB) to simulate the ce ...

Membrane proteins exist in a lipid bilayer and provide for cell–cell communication, transport solutes, and convert energies. Detergents are used to extract membrane proteins and keep them in solution for purification and subsequent analyses. The atomic force microscope (AFM) is a powe ...

The use of manganese ions (Mn2+) as an MRI contrast agent was introduced over 20 years ago in studies of Mn2+ toxicity in anesthetized rats (1). Manganese-enhanced MRI (MEMRI) evolved in the late nineties when Koretsky and associates pioneered the use of MEMRI for brain activity measurements (2) as we ...

Diffusion tensor MRI (DT-MRI) is the only non-invasive method for characterising the microstructural organization of tissue in vivo. Generating parametric maps that help to visualise different aspects of the tissue microstructure (mean diffusivity, tissue anisotropy and dom ...

Medical imaging has been transformed by a move from qualitative to quantitative approaches where image processing is used to enhance visual information and image analysis is used to derive structural and functional measurements. The ideal quantitative analysis methods are autom ...

Underlying the exquisite soft tissue contrast provided by magnetic resonance imaging are the inherent biophysical processes of relaxation. Through the intricate relationships between tissue microstructure and biochemistry and the longitudinal and transverse relaxa ...

Methods for quantitative imaging of the brain are presented and compared. Highly precise and accurate mapping of the absolute water content and distribution, as presented here, requires a significant number of corrections and also involves mapping of other MR parameters. Here, either T 1 a ...

The safe operation of both clinical and pre-clinical MR systems is critical. There are a wide range of potential MR hazards. This chapter covers both the theoretical background to issues of MR safety and the guidance on more practical issues. The main sources of information on national and interna ...

MRI offers the ability to visualise and measure blood flow in the human body non-invasively. MR angiography (MRA) provides images of the arterial blood vessels within the body and allows measurement of blood velocities along these arteries. Arterial spin labelling (ASL) is a method for measu ...

The combination of electroencephalography (EEG) with functional magnetic resonance imaging (fMRI) forms a powerful tool for the investigation of brain function, but concurrent implementation of EEG and fMRI poses many technical challenges. Here, the motivation for combining E ...

Functional magnetic resonance imaging (fMRI) has had an essential role in furthering our understanding of brain physiology and function. fMRI techniques are nowadays widely applied in neuroscience research, as well as in translational and clinical studies. The use of animal models in f ...