遗传学实验技术

Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (284 ...

1-cell embryo transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - those that have been damaged during t ...

The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host ...

General Comments Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µ ...

This is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately I can't attribute much of the development work since I was general ...

2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2 ...

The number of infertile couples has increased in recent years and is now estimated at 15–20% of all couples worldwide. Since its introduction into reproductive medicine in 1978 in vitro fertili ...

The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor ...

Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl2 up to 100 ml with Q store at room temperature 1M KPO4 pH 7.5 250 ml 1M KH2PO4 (monobasic) 68.05 g up ...

INTRODUCTION Phosphoproteins and peptides can be bound with high specificity to immobilized metal ions such as Fe3+ Ni2+ and Ga3+. This technique can be used with either on-line or off-line MS analysi ...

1、一般培养-保持胚胎干细胞处于未分化状态 培养基细胞复苏冻存细胞明胶包被细胞传代 2 、体外分化 培养基：包被有多聚鸟氨酸/纤维结合蛋白的培养板（使用或不使用盖玻片） 体外分化方法 注：以下培养针对于小鼠的R1胚胎干细胞系，其它胚胎干细胞的培养可以参考。不过人的胚胎干细胞培养不可以采用下面的protocol，需要用专用的protocol和培养基。 一般培养--维持ES细胞处于未分化状态 ES细胞 ...

组织原位杂交已广泛应用于植物组织、细胞的基因时空表达研究。 目前主要有3种方法：一是常规组织切片原位杂交，广泛适用于各种组织；二是大量细胞的整体原位杂交，已应用于花粉管和藻类合子；三是微量细胞的整体原位杂交，用于分离胚囊。整体原位杂交具有易操作、周期短、整体效果明显等特点，是一种较新的组织原位杂交方法。胚胎是个体发育的最初阶段，整体原位杂交作为检测胚胎发育过程中基因表达整体效果的快速、有效手段，已 ...

采用OG05 差异显示技术，分析水稻稻瘟病抗源材料“ 地谷”叶片受稻瘟病菌侵染前后的基因的表达差异，获得87个差异片段。对这87个差异片段进行了回收、重扩增与克隆，并对其中的81个片段进行了杂交鉴定。斑点杂交结果证实其中6个片段受稻瘟病菌诱导表达。进一步克隆测序并进行数据库比对分析表明其中一个与水稻4号染色体中一推测的苹果酸合成酶高度同源，一个与水稻11号染色体上的RPR1 ...

All living organisms have thousands to tens of thousands of unique genes encoded in their genome of which only a small fraction perhaps 15% are expressed in any individual cell. Therefore it is the te ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

The concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to systematically amplify and visualize most of the mRNA in ...

Assemble the following components: •Cellsgrown in 10 cm dishes •Room Temp.PBS •Cold PBS •Cold Sonication Buffer (1% SDS10 mM EDTA50 mM Tris-HCl pH 7.5) Prepare sonication buffe ...

Upstate公司在世界上最先开发出步骤优化简洁的商业化ChIP分析试剂盒，保证实验的准确性和可重复性，同时Upstate提供广泛可行的ChIP级别应用抗体。ChIP基本试剂盒和特定蛋白 质ChIP级别应用抗体结合完成一个完整的染色质免疫沉淀分析。在过去的数年中，Upstate提供的ChIP分析试剂盒和ChIP级别应用抗体得到了全世界科学家的认可，文献应用极为广泛。 Upstate最新推出EZ C ...

Reagents: ChIP sonication Buffer (1% Triton X-1000.1% Deoxycholate50 mM Tris 8.1150 mM NaCl5 mM EDTA): 10 ml 10% Triton X-100 1 ml 10% Deoxycholate 5 ml 1 M Tris-Cl pH 8.1 1 ml 0.5 M EDTA 3 ml 5 ...

Yeast Chromatin Immunoprecipitation (ChIP) Hahn Lab July 2003 Chromatin IP Method Day 1 1.Start 5 ml o/n cultures of strains to be tested Day 2 1.Inoculate 50 ml of the desired media with a volume of ...