遗传学实验技术

The purity of the transgene DNA used for microinjection is critical for the successful production of transgenic founder mice. DNA impurities in the form of bacterial endotoxins or organic contaminants ...

1.Restrict DNA and gel purify Cut 100ug DNA removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of ...

The success of DNA purification can have profound consequences on the success or failure of any injection effort. This summary of techniques from several different sources was compiled by Brad Preston ...

We have found the UltraClean™ GelSpin™ Kit (available from Mo Bio Laboratories Catalog number 12400-250) is a simple and fast way to obtain microinjection quality DNA. This is not to exclu ...

1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electro ...

Buffer composition is 10 mM Tris-HCl pH 7.5 0.1 mM EDTA 30 microM spermine 70 microM spermidine 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact unfragmented DNA molecule ...

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完整的实验方法请点击下面的链接下载： ...

完整的实验方法请点击下面的链接下载： ...

完整的实验方法请点击下面的链接下载： ...

The manipulation of embryonic stem (ES) cells to generate targeted mutations via homologous recombination has proved an invaluable resource for researchers from fields as diverse as embryology immunol ...

Introduction The recent discovery of RNA interference and in particular the observation that siRNAs can modulate gene expression at the level of transcription i.e. small-interfering RNA (siRNA) direc ...

Introduction Prolonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor trichostatin A specifically affects pericentric heterochromatin. Relocation of these ...

10 ul reactions amounts per reaction Make MM for 1 extra reaction every 6 ...

Introduction PCR protocols to detect rare deletions in DNA from complex populations of mutagenized worms typically depend on a large size differential between the wild-type product and the deletion p ...

Overview Our protocol for generating deletion mutations is based on the procedures of Bob Barstead and Gary Moulder at the Oklahome Medical Research Foundation in Oklahoma City. The procedure is to m ...

1、 选取7~8周龄雌性小鼠，阴道口封闭，作为供体，下午3:00左右，每只小鼠腹腔注射PMSG（10 IU）。 2、 47~48小时后，每只小鼠腹腔注射HCG（0.8 IU），并与正常公鼠合笼；另取数只适龄母鼠（2月龄以上）作为受体，阴道口潮红，与结扎公鼠合笼。 3、第二天上午9:00前观察供体、受体，有精栓者拿出备用。受体笼拿出作好隔离措施。 4、10:30左右，断颈处死供体，手术取出整个 ...

1. Introduction. This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene t ...

Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Aust ...

Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra ...