遗传学实验技术

1. Cut the required amount of DNA (banded on a CsCl gradient) with the appropriate restriction enzyme and check for complete digestion by running 500 ng on a minigel. The DNA concentration should be n ...

Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at 6 ...

Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at ...

Bleed mice: Obtain 300-400µl from mice of various ages (older mice that have been in the room for an extended period) and strains (include nude mice if in the room). 5-8 mice is a good number. ...

Injection and Holding Pipettes The glass capillary tubing used should be thin walled borosilicate glass without a fibre. e.g. Clark Electromedical Instruments ...

These protocols have come from the transgenic mouse list and are intended as a guide none of them have been tried in this lab Cell(s) transferred in small volume of water to a .5 ml tube and lysed by ...

This method is modified from Dr. Lyn Corcoran (WEHI)

This technique appeared as an article in Biotechniques Vol.14 No.3 pp412-414. This article is a report by Kristian Gundersen Theresa Hanley and John Merlie on the use of micropipette bevelling to incr ...

Culture should be 2 days old and colonies should be of medium to large size. This is important as cells need to be in log growth phase. Two small (25cm2) flasks are required one is harvested in morni ...

Pre-sterilize two pairs of fine forceps one pair iris scissors and one blunt-ended 23 gauge needle. Take 3.5 day pregnant mouse kill and dissect out entire uterus using sterile technique. Place uteru ...

Before you start have ready the following: - Sterilize 2 pairs of curved forceps 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES with an 18 ...

Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra ...

Solutions

This method works well for 17.5 day mouse embryos. All stains should be made up fresh. Fix embryos in 90% ethanol for at least 1 week longer if possible. Prior to staining remove skin and viscera pa ...

The aggregation method for generating chimaeras as opposed to the microinjection technique is useful as it does not require expensive microinjection apparatus or sophisticated manipulative skills and ...

Blastocyst transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - the embryos should recavitate if they ha ...

完整的实验方法请点击下面的链接下载： ...

DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载： ...

Mouse embryos staining including: in vitro transcription of riboprobe embryo fixation and permeabilization hybridization and post-hybridization washes immunodetection and development of signal. 完整的实验 ...

FGFR-AP-FGF Binding Assay PCR Assays for Knockout Mice RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of prote ...