生物信息学技术

Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA to be purified to a microfuge tube or tubes.b) pl ...

AcknowledgementsThe organizer of the workshop acknowledges Dr. Murray Milford Professor and Interim Head and Dr. Mark Hussey Professor and Interim Associate Head Department of Soil and Crop Sciences ...

This protocol is designed to allow the use of 96-well trays (8x12 microtiter tray format) and multi-channel devices from the initial inoculation step to the final cycle sequence reaction. It will yie ...

(Protocol for a single high-density BAC colony filter (HDR filter) of 22 cmx22 cm) to be screened using Amersham''s ECL hybridization kit; this protocol was kindly contributed by Dr. Kazuei Mita from ...

I. Introduction: This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked ...

我积攒了大量的样本，如何能快速转化成具有临床意义的数据用于发表? 我要在现有的临床样本中发现肿瘤标志物，用于指导临床治疗，应该怎么做？ 我的临床样本量太少了，现有的技术无法支持多个指标的检测，怎么办？ 当您遇到这些问题的时候，实际上您碰到的是困扰多数医师的技术难题：如何快速的将临床样本转化为学术课题或创新的临床应用项目。如何打破这个瓶颈，快速获得有效的数据，这就是目前转化医学领域最受关注的 ...

45Ca2+ uptake study in PC12 cells1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study. 2. Rinse the cells with 1 ml secretion buffer (150 mM NaCl 5mM KCl 2mM CaCl2 ...

OUTLINEAlthough in the Review Article : "Idiopathic Thrombocytopenic Purpura: A practice Guideline Developed by Explicit Methods for the American Society of Hematology" bleeding time is considered as ...

Blood Smear: Preparation and StainingReference:Davidson I. and Henry J. Clinical Diagnosis by Laboratory Methods I. Davidsohn and J. Henry eds. W.B. Saunders Co. Philadelphia 1974 pp. 135-137. Wintrob ...

OUTLINEAbnormal Clot Retraction indicates on abnormal platelet numbers abnormal glycoprotein amount or structure abnormal platelet signaling abnormal fibrinogen levels or abnormal fibrinogen structure ...

OUTLINEAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay. PROTOCOLResuspend 1.6x10^8 of CMFDA-labeled platelets in 200µl of BSGC-BSA (v/w 1%) either with a c ...

OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) ( ...

OUTLINEThe erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts.PRO ...

OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible! This protocol is based on differential centrifugation th ...

OUTLINEPlatelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and mainly of i ...

Serum Separation from Whole Blood1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot. 2) Leave sample at 4°C overnight to allow the clot to contract. 3) Usin ...

OUTLINEWestern blot is a wide used technique to identify a target protein/s for the certain antibody.PROTOCOLPrepare platelets. Lyse washed platelets (109 platelets/ml) with 1ml of ice-cold complete l ...

OUTLINECMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it can be introduced into live (!) cells. Once inside ...

1 BackgroundSummarize the purpose and objectives of the tissue bank in this section.2 Eligibility to Participate in the Research Tissue BankThis section should specify who is eligibl ...

OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) ( ...