生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract The conversion of 3?,5??disilylated 2??O?(methylthiomethyl)ribonucleosides to 2??O?(phthalimidooxymethyl)ribonucleosides is achieved in

Abstract Table of Contents Materials Figures Literature Cited Abstract High?content functional protein microarrays, such as ProtoArray Human Protein Array from Life Technologies, contain thousands of prot

Abstract Table of Contents Materials Figures Literature Cited Abstract In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein?protein interactions in situ

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the ChIP?exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion f

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for quantifying various cellular features (e.g., volume, total and subcellular fluorescence localization)

Abstract Table of Contents Materials Figures Literature Cited Abstract Proteases have garnered interest as candidate biomarkers and therapeutic targets for many human diseases. A key challenge is the iden

Abstract Table of Contents Materials Figures Literature Cited Abstract The unique reactivity and relative rarity of cysteine among amino acids makes it a convenient target for the site?specific chemical m

Abstract Table of Contents Materials Figures Literature Cited Abstract Protocols for the synthesis of oligodeoxynucleotides with a short peptidyl substituent linked to the 5??O?terminus through a phosphodie

Abstract Table of Contents Materials Figures Literature Cited Abstract One?bead?one?compound (OBOC) libraries consist of structurally related compounds (e.g., peptides) covalently attached to a solid supp

Abstract Table of Contents Materials Figures Literature Cited Abstract Deubiquitinating enzymes (DUBs) reverse the process of ubiquitination, and number nearly 100 in humans. In principle, DUBs represent

Abstract Table of Contents Materials Figures Literature Cited Abstract To be secreted or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the G

Abstract Table of Contents Materials Figures Videos Literature Cited Abstract Methods useful for exploring the formation and functions of primary cilia in living cells are

Abstract Table of Contents Figures Literature Cited Abstract Methods to quantify features of individual cells using light microscopy have become widely used in biology. A multitude of computational tools has been developed for image analys

Abstract Table of Contents Figures Literature Cited Abstract Modern computational strategies have allowed for the direct modeling of the sedimentation process of heterogeneous mixtures, resulting in sedimentation velocity (SV) size?distrib

Abstract Table of Contents Literature Cited Abstract The physicochemical and biopharmaceutical properties of putative drug molecules impact their performance in both in vitro and in vivo studies. The design and selection of molecules with drug?like properties assists in the

Abstract Table of Contents Materials Figures Literature Cited Abstract Commercial N2?isobutyryl?5??O?(4,4??dimethoxytrityl)?2??O?(propargyl)guanosine is converted to its 3??O?levulinyl ester in a yield of 9

Abstract Table of Contents Materials Figures Literature Cited Abstract 5??Triphosphates are building blocks for enzymatic synthesis of DNA and RNA. This unit presents a protocol for convenient synthesis o

Abstract Table of Contents Materials Figures Literature Cited Abstract Low?affinity extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by me

Abstract Table of Contents Materials Figures Literature Cited Abstract Protein microarrays allow unique approaches for interrogating global protein interaction networks. Protein arrays can be divided into

Abstract Table of Contents Materials Figures Literature Cited Abstract Functional protein microarrays offer the capability for high?throughput protein interaction analysis and have long promised to be a p